Photon Migration Codes for Tissue Spectroscopy & Imaging

组织光谱学的光子迁移代码

• 批准号: 7468375
• 负责人: Mary-Ann Mycek
• 金额: $ 20.44万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-14 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7468375
• 关键词: Applications Grants; Characteristics; Clinical; Code; Collection; Communities; Compatible; Complex; Computer software; Contrast Media; Data; Diagnostic; Disease; Electronics; Endoscopy; Ensure; Fiber Optics; Goals; Image; Imagery; Immigration; Internet; Invasive; Light; Link; Measurement; Methods; Modeling; Modification; Molecular; Morphology; NIH Program Announcements; Optical Methods; Optics; Pharmacologic Substance; Photons; Physiological; Property; Recording of previous events; Research Personnel; Resources; Signal Transduction; Simulate; Site; Source; Spectrum Analysis; Staging; Techniques; Testing; Time; Tissues; absorption; design; detector; digital; in vivo; innovation; instrumentation; migration; multidisciplinary; novel; platform-independent; pre-clinical; programs; theories; tool

DESCRIPTION (provided by applicant): Methods of optical spectroscopy and imaging are being developed for biomedical applications in non- or minimally invasive tissue diagnostics, including sensing molecular concentrations of delivered pharmaceutical or contrast agents, probing tissue physiologic status, and detecting early stages of disease in vivo. In tissue optics, one of the challenges is to obtain quantitative information despite the detected signal variability introduced by tissue morphology and optical absorption and scattering. The overall goal of this application is to develop, validate, and disseminate computational codes to numerically simulate photon migration characteristics useful for quantitative tissue spectroscopy and imaging. We will develop and employ innovative computational codes designed to model steady-state and time-resolved excitation and fluorescent light propagation in complex tissues approximately 102 -104 times faster than current methods. The computational resources developed here will be broadly applicable to problems in pre-clinical and clinical biomedical optical spectroscopy and imaging, including minimally-invasive applications requiring light delivery and collection via fiber-optic probe configurations. A multidisciplinary team of researchers with expertise in biomedical optics and clinical endoscopy has been assembled to ensure that these goals are achieved. The specific aims of this R01 grant proposal are: (1) To develop and numerically test novel computational codes for steady-state and time-resolved photon migration in tissues with complex morphologies and source-detector geometries; (2) To experimentally validate the computational codes developed in Aim #1; (3) To efficiently disseminate the computational codes developed in Aim #1 and validated in Aim #2 to the scientific community via download links and platform-independent user-interactive interfaces on the Pi's web-site. The innovative computational codes developed and validated here will be broadly applicable to a variety of problems in quantitative tissue optical spectroscopy and imaging. Rapid electronic dissemination via the internet will facilitate the generalized use of these digital tools, which will serve as a resource for a broad cross-section of researchers investigating problems in tissue optics.

描述（由申请人提供）：正在为非侵入性组织诊断的生物医学应用开发光谱和成像方法，包括传感的药物或对比剂的分子浓度，探测组织生理状态，并检测VIVO疾病的早期阶段。在组织光学器件中，尽管检测到组织形态，光吸收和散射引入的信号变异性，但挑战之一是获得定量信息。该应用的总体目标是开发，验证和传播计算代码，以模拟对定量组织光谱和成像有用的光子迁移特性。我们将开发和采用创新的计算代码，旨在模拟复杂组织中的稳态和时间分辨的激发和荧光传播，比当前方法快102-104倍。此处开发的计算资源将广泛适用于临床前和临床生物医学光谱和成像的问题，包括最小的侵入性应用，需要通过光纤探针构型进行光输送和收集。已经组装了一支具有生物医学光学和临床内窥镜检查专业知识的研究人员的多学科团队，以确保实现这些目标。该R01赠款提案的具体目的是：（1）开发和数值测试新的计算代码，以在具有复杂形态和源探测器几何形态的组织中进行稳态和时间分辨的光子迁移； （2）实验验证AIM＃1中开发的计算代码； （3）有效地传播了AIM＃1中开发的计算代码，并在AIM＃2中通过下载链接和无独立的PIE网站网站上的无独立的用户相互作用接口对AIM＃2进行了验证。此处开发和验证的创新计算代码将广泛适用于定量组织光谱和成像的各种问题。通过Internet进行快速的电子传播将有助于这些数字工具的广义使用，这将作为研究组织光学问题的广泛研究人员的资源。

项目成果

Image restoration for fluorescence lifetime imaging microscopy (FLIM).

• DOI: 10.1364/oe.16.019192
• 发表时间: 2008-11
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Dhruv Sud;M. Mycek

Calibration and validation of an optical sensor for intracellular oxygen measurements.

用于细胞内氧测量的光学传感器的校准和验证。

• DOI: 10.1117/1.3116714
• 发表时间: 2009
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Sud,Dhruv;Mycek,Mary-Ann
• 通讯作者: Mycek,Mary-Ann

Instrumentation to rapidly acquire fluorescence wavelength-time matrices of biological tissues.

快速获取生物组织荧光波长-时间矩阵的仪器。

• DOI: 10.1364/boe.1.000574
• 发表时间: 2010
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Lloyd,WilliamR;Wilson,RobertH;Chang,Ching-Wei;Gillispie,GregoryD;Mycek,Mary-Ann
• 通讯作者: Mycek,Mary-Ann

Total variation versus wavelet-based methods for image denoising in fluorescence lifetime imaging microscopy.

• DOI: 10.1002/jbio.201100137
• 发表时间: 2012-05
• 期刊: JOURNAL OF BIOPHOTONICS
• 影响因子: 2.8
• 作者: Chang, Ching-Wei;Mycek, Mary-Ann
• 通讯作者: Mycek, Mary-Ann

Photon-tissue interaction model enables quantitative optical analysis of human pancreatic tissues.

• DOI: 10.1364/oe.18.021612
• 发表时间: 2010-10-11
• 期刊: Optics express
• 影响因子: 3.8
• 作者: Wilson RH;Chandra M;Chen LC;Lloyd WR;Scheiman J;Simeone D;Purdy J;McKenna B;Mycek MA
• 通讯作者: Mycek MA