Glucose Signal Transduction Pathways in Plants

植物中的葡萄糖信号转导途径

• 批准号: 6921533
• 负责人: JEN SHEEN
• 金额: $ 36.31万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6921533
• 关键词: Arabidopsis; biological signal transduction; functional /structural genomics; gene environment interaction; gene expression; gene expression profiling; gene mutation; genetically modified plants; glucose; glucose metabolism; molecular genetics; phosphorylation; plant genetics; plant proteins; protein kinase; protein structure function; proteomics; regulatory gene

DESCRIPTION (provided by applicant): Glucose is a universal nutrient preferred by most organisms and serves essential roles in energy supply, carbon storage, biosyntheses, and carbon skeleton and cell wall formation. The ability to sense glucose and its deprivation is of fundamental importance in organisms as diverse as E. coli, yeast, flies, mammals, and plants. The significance of glucose as a direct and central signaling molecule in multi-cellular animals and plants has only been recently recognized. In plants whose life revolves around sugar production through photosynthesis, glucose has emerged as a key regulator of many vital processes, including embryogenesis, germination, seedling development, root, stem and shoot growth, carbon and nitrogen metabolism, reproduction, stress responses, and senescence. Our goal is to understand how plants sense glucose signals to control gene expression and modulate growth and development. Studies of glucose responses in plants will enhance our general understanding of glucose signaling mechanisms conserved in other eukaryotes from yeast to mammals. Complementary genomic, proteomic, genetic, molecular, biochemical, and cellular strategies and tools will be used to study glucose signaling pathways in Arabidopsis thaliana as a plant model. Four specific aims are: Aim 1. Genome-wide identification and molecular genetic analysis of glucose responsive genes. We will combine the power of glucose signaling mutants and global gene expression profiling to identify glucose responsive genes and perform functional analysis of selected target genes. Aim 2. Proteomic and genetic analysis of the HXK1 protein complexes in glucose signaling. We will identify the key components of the nuclear HXK1 protein complexes and investigate the function of novel regulatory genes in glucose signaling using genetic, cellular and biochemical tools. Aim 3. Genetic analysis of the gin2 suppressor (g/s) mutants. A genetic approach will be taken to identify novel genes involved in HXK1-mediated signaling. Aim 4. Genomic and reverse genetic analysis of glucose deprivation responses. We will study the functions of Arabidopsis protein kinases (KIN10 and KIN11) that control diverse genes in glucose deprivation and stress responses.

描述（由申请人提供）：葡萄糖是大多数生物体首选的通用营养素，在能量供应、碳储存、生物合成以及碳骨架和细胞壁形成中发挥重要作用。感知葡萄糖及其缺乏的能力对于大肠杆菌、酵母、果蝇、哺乳动物和植物等多种生物体至关重要。葡萄糖作为多细胞动物和植物中直接和中心信号分子的重要性直到最近才被认识到。在植物中，葡萄糖已成为许多生命过程的关键调节剂，包括胚胎发生、发芽、幼苗发育、根、茎和芽生长、碳和氮代谢、繁殖、应激反应和衰老。我们的目标是了解植物如何感知葡萄糖信号来控制基因表达并调节生长和发育。对植物中葡萄糖反应的研究将增强我们对从酵母到哺乳动物的其他真核生物中保守的葡萄糖信号传导机制的一般理解。互补的基因组、蛋白质组、遗传、分子、生化和细胞策略和工具将用于研究拟南芥作为植物模型的葡萄糖信号传导途径。四个具体目标是： 目标 1. 葡萄糖反应基因的全基因组鉴定和分子遗传学分析。我们将结合葡萄糖信号突变体和全局基因表达谱的力量来识别葡萄糖反应基因并对选定的目标基因进行功能分析。 目标 2. 对葡萄糖信号转导中的 HXK1 蛋白复合物进行蛋白质组学和遗传分析。 我们将鉴定核 HXK1 蛋白复合物的关键成分，并利用遗传、细胞和生化工具研究葡萄糖信号传导中新型调控基因的功能。 目标 3. gin2 抑制子 (g/s) 突变体的遗传分析。 将采用遗传方法来鉴定参与 HXK1 介导的信号传导的新基因。 目标 4. 葡萄糖剥夺反应的基因组和反向遗传分析。 我们将研究拟南芥蛋白激酶（KIN10 和 KIN11）的功能，这些蛋白激酶控制葡萄糖剥夺和应激反应中的多种基因。