Chloroplast to Nucleus Signaling by use of Tetrapyrroles

使用四吡咯进行叶绿体到细胞核的信号传导

• 批准号: 7121520
• 负责人: JESSE D WOODSON
• 金额: $ 4.6万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7121520
• 关键词: Arabidopsis; affinity chromatography; biological signal transduction; cell nucleus; chloroplasts; functional /structural genomics; gene expression; gene mutation; intracellular transport; magnesium; metalloporphyrins; northern blottings; open reading frames; plant genetics; plant proteins; porphyrin metabolism; postdoctoral investigator; protein structure function; tetrapyrroles; transport proteins

DESCRIPTION (provided by applicant): Retrograde signaling from organelles to the nucleus is an important part of the cellular signaling network and has been implicated in cell aging and tumor progression. This work plans to achieve a deeper understanding of these signaling pathways by investigating how plant chloroplasts send signals to the nucleus. Recently it has been shown that chlorophyll intermediates Protoporphyrin IX and Mg-Protoporphyrin IX, synthesized in the chloroplast, are signal inputs for at least two distinct pathways that regulate gene expression in the nucleus (phytochrome and GUN signaling pathways, respectively). Using the flowering plant Arabidopsis thaliana as a model system, the proposed studies will integrate genetic, molecular, as well as biochemical approaches to complete the following specific aims; (1) elucidate the mechanism(s) by which the accumulation of Mg-Protoporphyrin IX in the chloroplast regulates gene expression in the nucleus; (2) and determine the role of Protoporphyrin IX in phytochrome signaling and how its accumulation regulates nuclear gene expression. Completion of the proposed projects will contribute to the understanding of intracellular signaling, organelle communication, and how metabolic pathways and light can regulate gene expression.

描述（由申请人提供）： 从细胞器到细胞核的逆行信号是细胞信号网络的重要组成部分，与细胞衰老和肿瘤进展有关。这项工作计划通过研究植物叶绿体如何向细胞核发送信号来更深入地了解这些信号通路。最近的研究表明，在叶绿体中合成的叶绿素中间体原卟啉 IX 和镁原卟啉 IX 是至少两个调节细胞核内基因表达的不同途径（分别为光敏色素和 GUN 信号传导途径）的信号输入。使用开花植物拟南芥作为模型系统，拟议的研究将整合遗传、分子和生化方法来完成以下具体目标； (1) 阐明叶绿体中镁原卟啉 IX 的积累调节细胞核内基因表达的机制； (2)并确定原卟啉IX在光敏色素信号传导中的作用以及其积累如何调节核基因表达。完成拟议项目将有助于理解细胞内信号传导、细胞器通讯以及代谢途径和光如何调节基因表达。