Resistant E.coli in Humans and Poultry

人类和家禽中的耐药大肠杆菌

• 批准号: 6946917
• 负责人: JAMES R JOHNSON
• 金额: $ 26.53万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6946917
• 关键词: Escherichia coli; Escherichia coli infections; antibiotics; bacterial genetics; cephalosporins; clinical research; communicable disease transmission; consumer product; drug resistance; eating; epidemiology; feces; feces analysis; food contamination; food processing /preparation; genotype; human subject; phenotype; poultry products; public health; quinoline; sulfamethoxazole; trimethoprim; virulence

Background: Poultry products are the highest-risk foods for containing antibiotic-resistant and extraintestinal-pathogenic E. coli. Whether such strains or their resistance elements are actually transmitted to consumers and, if so, pose a significant human health threat, is unknown. Goals: Assess poultry contact and consumption as risk factors for human colonization with trimethoprim-sulfamethoxazole (TS)-, fluoroquinolone (FQ)-, and extended-spectrum cephalosporin (ESC)-resistant E. coli (i.e. TSREC, FQREC, and ESCREC), and define the degree of commonality among poultry-source versus human-source TSREC, FQREC, and ESCREC and their respective resistance elements. Plans: Temporally-matched TSREC, FQREC, and ESCREC isolates from fecal samples from 629 human volunteers in four rural Midwestem communities, 160 retail poultry items fi'om the same locales, and local clinical microbiology laboratories will be compared. Risk factor analysis wi.ll identify epidemiological predictors of human colonization with TSREC, FQREC, and ESCREC, including contact with and consumption of poultry. Detailed molecular analysis will define the relationships between poultry-source and human-source isolates and their respective resistance elements, plus clarify the virulence potential for humans of poultry-source E. coll. Relevance to the RFA: The proposal addresses Research Objective II of the RFA by helping implement Action Item #50 found in "A Public Health Action Plan to Prevent Antimic_:obial Resistance (Part I: Domestic Issues): Focus Area II, Prevention and Control": Conduct additional research to further define the effects of using various veterinary drugs on the emergence of resistant bacteria that infect or colonize food animals of different species, using various animal husbandry practices [indiirectly addressed]. Identify risk factors and preventive measures [addressed, for transmission to humans]. Assess the associated risk of. " Transmission of AR infections to humans [addressed]," Clinical disease in humans [addressed],' and Transfer of resistance factors from animal flora to human flora [addressed]. Does the research address a current and compelling problem of antimicrobial resistance that is of high public health importance and for which research is needed? [yes].

背景：家禽产品是含有抗生素耐药性和的最高风险食品 遗传性疾病大肠杆菌。这种菌株或它们的抵抗元素是否实际上是传输给消费者的，如果是的，则构成重大的人类健康威胁，这是未知的。 目标：将家禽接触和消费评估为人类与三甲氧苄啶 - 磺胺甲氧唑（TS） - 氟喹诺酮（FQ） - 和延长的谱谱头孢菌素（ESC） - 抗抗性大肠杆菌（即TSREC，FQREC和ESCREC）的危险因素（FQ） - po pltry po poul， TSREC，FQREC和ESCREC及其各自的抵抗元素。 计划：将在四个农村中西部社区中的629名人类志愿者，160个零售家禽物品以及同一地区以及当地临床微生物实验室中的160个零售家禽项目中的粪便样本中的暂时匹配的TSREC，FQREC和ESCREC分离株进行比较。危险因素分析WI.LL会确定人类与TSREC，FQREC和ESCREC的人类定殖的流行病学预测因子，包括与家禽的接触和消费。详细的分子分析将定义家禽源与人类源分离株及其各自的抗性元素之间的关系，并阐明家禽源人类的毒力潜力。 与RFA的相关性：该提案通过帮助实施“预防公共卫生行动计划中的行动项目＃50”来解决RFA的研究目标II，以防止反抗药性：抗抗病性（第一部分：国内问题）：焦点区域II，预防和控制”：进行其他研究以进一步定义使用各种兽医药物对各种动物的影响，以造成各种动物的影响，以造成各种动物的影响，以造成各种动物的锻炼，以造成各种动物的态度，以相同的态度造成群体的出现。解决]。确定风险因素和预防措施[解决，以传播给人类]。评估相关的风险。 “将AR感染传播给人类[已解决]”，人类的临床疾病[已解决]和转移 从动物菌群到人类植物群的抗性因素[已解决]。该研究是否解决了当前且引人注目的抗菌耐药性问题，该问题具有很高的公共健康重要性以及需要哪种研究？ [是的]。

项目成果

Antimicrobial drug-resistant Escherichia coli from humans and poultry products, Minnesota and Wisconsin, 2002-2004.

• DOI: 10.3201/eid1306.061576
• 发表时间: 2007-06
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Johnson JR;Sannes MR;Croy C;Johnston B;Clabots C;Kuskowski MA;Bender J;Smith KE;Winokur PL;Belongia EA
• 通讯作者: Belongia EA