Core--Microscopy

核心--显微镜

• 批准号: 6967722
• 负责人: DAVID L. BRAUTIGAN
• 金额: $ 24.08万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6967722
• 关键词: biological transport; biomedical facility; confocal scanning microscopy; electron microscopy; image processing; intracellular transport; light microscopy; microfilaments; myosins; nuclear transport; protein localization; protein structure; protein transport; vascular smooth muscle

The Core will provide electron and light microscopy imaging that is essential to accomplishing the goals of the individual projects. The intraceltular localization of proteins, nucleo-cytoplasmic transport of transcription factors, and imaging of myosin filaments are key to testing the hypotheses of this Program, and will require extensive use of confocal microscopy and electron microscopy. The core will provide training for individual investigators, align and calibrate these instruments, assist with preparation of specimens, and oversee scheduling of experiments and maintenance. The core includes a panel of local microscopy experts, who will conduct training and implement the latest upgrades and innovations. The Core staff wilt ensure that the Program gets effective and efficient use of these resources. A common core for microscopy brings with it other benefits in terms of sharing of protocols and reagents, trading of information on experiments in progress and nurturing a sense of camaraderie between individuals working on different projects, as they use common facilities and methodology to study related issues.

该核心将提供电子和光学显微镜成像，这对于实现各个项目的目标至关重要。蛋白质的细胞内定位、转录因子的核细胞质运输以及肌球蛋白丝的成像是检验该计划假设的关键，并且需要广泛使用共聚焦显微镜和电子显微镜。该核心将为个别研究人员提供培训，调整和校准这些仪器，协助准备样本，并监督实验和维护的安排。该核心包括一个由当地显微镜专家组成的小组，他们将进行培训并实施最新的升级和创新。核心人员将确保该计划 有效且高效地利用这些资源。显微镜的共同核心带来了其他好处，包括共享实验方案和试剂、交换正在进行的实验的信息以及培养从事不同项目的个人之间的友情，因为他们使用共同的设施和方法来研究相关问题。