National Resources for Aplysia

海兔国家资源

• 批准号: 6898466
• 负责人: Patrick Joseph Walsh
• 金额: $ 46.16万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6898466
• 关键词: Aplysia; algae; alternatives to animals in research; animal breeding; animal care; animal colony; animal population genetics; biological models; developmental genetics; developmental neurobiology; functional /structural genomics; genetic strain; genetically modified animals; life cycle; membrane channels; microarray technology; molecular biology information system; neurophysiology; technology /technique development; voltage /patch clamp

DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food, Gracilaria sp.) to NIH-sponsored researchers. Aplysia californica is an important non-vertebrate (Opisthobranch mollusc) model system for health-related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research, and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production-We anticipate increasing our production of animals by approximately 25% per year during this next phase of funding and will make specimens from all life stages available at a price competitive with fieldcollected specimens. 2. Functional Genomics-We will undertake a cDNA/EST sequencing project of neural and other tissues from different developmental stages (larvae, juveniles, and reproductive and senescent animals), and use these cDNAs to produce microarrays. Following appropriate validation and quality control, these microarrays will be available for at-cost purchase by our user community, and will also be used to examine gene expression changes in our hatchery population during different developmental stages, and after exposure to other experimental conditions including variable temperature, egg-laying hormone administration, etc. All sequence and array experimentation data will be made available through web-based database access. 3. Developmental Neurophysiology- We will continue study of ion current modulation in the bag cells observed to vary with development, genetic background, and between hatchery reared and wild caught animals. Understanding electrophysiological correlates of growth and maturation are important to the Resource because they may affect the interpretation of data by users of the Resource. A correlative study will examine how different growth rates of the animals produce variations in development times for the nervous system changes that culminate in sexual maturity. 4. Animal Health Monitoring-We will continue a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. Through this combination of production and basic research, we will improve the model system and extend its usefullness to other areas of research.

描述（由申请人提供）： 该提案描述了全国Aplysia资源的延续，其总体目标是向NIH赞助的研究人员提供一致的高质量培养的Sea Hares Aplysia加利福尼亚（及其培养的红色藻类食品，Gracilaria sp。）。 Aplysia Californica是与健康相关研究的重要非掌管脑（Opisthobranch Mollusc）模型系统，主要用于行为和学习的神经生理学。我们将继续生产动物进行研究，并进行旨在探索新模型用途并改善资源的基础研究。有四个子项目/特定目的：1。生产我们预计，在下一个资金阶段，我们的动物的产量每年增加约25％，并且将使所有生命阶段的标本都以与现场收获的标本的价格竞争。 2。功能性基因组学我们将从不同的发育阶段（幼虫，少年以及生殖和衰老动物）进行神经和其他组织的cDNA/EST测序项目，并使用这些cDNA产生微阵列。遵循适当的验证和质量控制后，这些微阵列将由我们的用户社区购买，并且还将用于检查不同发育阶段的孵化场的基因表达变化，并在暴露于其他实验条件（包括变量）的其他实验条件下进行。温度，卵激素给药等。将通过基于Web的数据库访问提供所有序列和阵列实验数据。 3。发育性神经生理学 - 我们将继续研究观察到的离子电流调节，观察到随发育，遗传背景以及孵化场饲养和野生捕获的动物之间的变化。了解生长和成熟的电生理相关性对资源很重要，因为它们可能会影响资源用户对数据的解释。一项相关研究将研究动物的不同生长速率如何产生发育时间的变化，导致性成熟度的最终变化。 4.动物健康监测 - 我们将继续基于筛查水质和动物健康参数的监测计划，以确保在孵化场的任何动物发育阶段可能发生的任何疾病过程的快速检测和完整记录。任何观察到的疾病综合征和可疑病原体都将进行研究，并采用适当的控制措施。通过生产和基础研究的结合，我们将改善模型系统，并将其有用性扩展到其他研究领域。