NATIONAL RESOURCE FOR APLYSIA

海兔国家资源

• 批准号: 6188396
• 负责人: Patrick Joseph Walsh
• 金额: $ 33.06万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188396
• 关键词: Aplysia; algae; alternatives to animals in research; animal breeding; animal care; animal colony; animal population genetics; developmental neurobiology; genetic strain; genetically modified animals; Aplysia; algae; alternatives to animals in research; animal breeding; animal care; animal colony; animal population genetics; developmental neurobiology; genetic strain; genetically modified animals

This proposal describes the establishment of a National Resource for Aplysia, whose overall goal is to provide consistently high quality cultured sea hares aplysia californica (and their cultured red algae food Gracilaria sp.) To NIH sponsored researchers. A. Californica is an important non- vertebrate (opisthobranch mollusc) model system for health related research, in the neurophysiology of behavior and learning. We will produce animals for research, and will conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-project/specific aims. 1. Production- We will increase our projected 1994 production by approximately 225% over the tenure of this grant and will make specimens from all life stages available at a price competitive with field-collected specimens. 2. Genetics- We will perform population genetic studies to characterize the amount of genetic variation present in our hatchery population in comparison to natural populations. The use of this genetic information will be to provide NIH-sponsored investigators with heterozygosity data for the hatchery population and with genetic markers for desirable/undesirable traits. 3. Developmental Neurophysiology-This project will investigate the regulation of neuronal excitability affecting reproductive development. The single cell voltage clamp technique will be applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in neurosecretory bag cells. Development of this modulatory capability is an integral part of sexual maturation. This sub-project will further characterize the neurophysiology of immature A. Californica, potentially leading to a new model use, and will yield information useful to hatchery maturation and breeding strategies. 4. Animal Health Monitoring-We will establish a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animal at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied.

该提案描述了建立国家资源 Aplysia的总体目标是提供一贯的高质量 培养的海野腹腹泻（及其培养的红藻类 食品Gracilaria sp。）NIH赞助的研究人员。 A. Califortica是一个 重要的非脊椎动物（Opisthobranch Mollusc）模型系统 与健康有关的研究，行为神经生理学和 学习。 我们将生产动物进行研究，并将进行基本 旨在探索新模型使用并改善的研究 资源。 有四个子项目/特定目标。 1。生产 - 我们 将使我们预计的1994年产量增加约225％ 这笔赠款的任期，并将从所有生命阶段制造标本 与现场收集的标本具有竞争力的价格。 2。 遗传学 - 我们将进行人群遗传研究以表征 我们孵化场中存在的遗传变异量 与自然种群进行比较。 使用此遗传信息 将为NIH赞助的研究者提供杂合性数据 对于孵化场的种群，并带有遗传标记 理想/不良特征。 3。发育性神经生理学 - 这 项目将调查神经元兴奋性的调节 影响生殖发展。 单细胞电压夹 技术将应用于研究的发展 调节性刺激的能力在应用于离子电流的能力上 调节刺激能力发展的研究 作用于神经分泌袋细胞中的离子电流。 发展的发展 调节能力是性成熟的组成部分。 这 亚项目将进一步表征未成熟的神经生理学。 加州，有可能导致新的模型使用，并将产生 对孵化场成熟和育种策略有用的信息。 4。 动物健康监测 - 我们将基于 筛选水质和动物健康参数以确保快速 检测和完整的文档，了解可能的任何疾病过程 发生在孵化场的动物的任何发育阶段。 任何 将研究疾病综合征和可疑病原体 并采用适当的控制措施。