NATIONAL RESOURCE FOR APLYSIA

海兔国家资源

• 批准号: 6188396
• 负责人: Patrick Joseph Walsh
• 金额: $ 33.06万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188396
• 关键词: Aplysia; algae; alternatives to animals in research; animal breeding; animal care; animal colony; animal population genetics; developmental neurobiology; genetic strain; genetically modified animals

This proposal describes the establishment of a National Resource for Aplysia, whose overall goal is to provide consistently high quality cultured sea hares aplysia californica (and their cultured red algae food Gracilaria sp.) To NIH sponsored researchers. A. Californica is an important non- vertebrate (opisthobranch mollusc) model system for health related research, in the neurophysiology of behavior and learning. We will produce animals for research, and will conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-project/specific aims. 1. Production- We will increase our projected 1994 production by approximately 225% over the tenure of this grant and will make specimens from all life stages available at a price competitive with field-collected specimens. 2. Genetics- We will perform population genetic studies to characterize the amount of genetic variation present in our hatchery population in comparison to natural populations. The use of this genetic information will be to provide NIH-sponsored investigators with heterozygosity data for the hatchery population and with genetic markers for desirable/undesirable traits. 3. Developmental Neurophysiology-This project will investigate the regulation of neuronal excitability affecting reproductive development. The single cell voltage clamp technique will be applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in neurosecretory bag cells. Development of this modulatory capability is an integral part of sexual maturation. This sub-project will further characterize the neurophysiology of immature A. Californica, potentially leading to a new model use, and will yield information useful to hatchery maturation and breeding strategies. 4. Animal Health Monitoring-We will establish a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animal at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied.

该提案描述了建立国家资源 Aplysia，其总体目标是提供始终如一的高质量 养殖海兔加利福尼亚海兔（及其养殖红藻） 食品 Gracilaria sp.）致 NIH 资助的研究人员。 A. 加利福尼亚州是 重要的非脊椎动物（后鳃类软体动物）模型系统 健康相关的研究，行为的神经生理学和 学习。 我们将生产用于研究的动物，并将进行基础研究 研究旨在探索新模型的用途并改进 资源。 有四个子项目/具体目标。 1. 生产-我们 将使我们 1994 年的预计产量比 这笔赠款的期限并将制作所有生命阶段的标本 与现场采集的标本相比，其价格具有竞争力。 2. 遗传学-我们将进行群体遗传学研究来表征 我们的孵化场种群中存在的遗传变异量 与自然种群的比较。 该遗传信息的利用 将向 NIH 资助的研究人员提供杂合性数据 用于孵化场群体并带有遗传标记 理想/不理想的特征。 3. 发育神经生理学-这 项目将研究神经元兴奋性的调节 影响生殖发育。 单节电池电压钳 技术将被应用于研究发展 调节刺激作用于离子电流的能力 调节刺激能力发展的研究 作用于神经分泌袋细胞中的离子电流。 开发此 调节能力是性成熟的一个组成部分。 这 子项目将进一步表征未成熟 A 的神经生理学特征。 Californica，可能会导致新模型的使用，并将产生 对孵化场成熟和育种策略有用的信息。 4. 动物健康监测-我们将根据以下情况制定监测计划 筛查水质和动物健康参数，以确保快速 检测并完整记录任何可能发生的疾病过程 发生在孵化场动物的任何发育阶段。 任何 将调查观察到的疾病症状和可疑病原体 并采取适当的控制措施。