Core--Behavioral Assessment

核心--行为评估

• 批准号: 7090247
• 负责人: John J. Hablitz
• 金额: $ 17.17万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7090247
• 关键词: behavior test; behavioral /social science research tag; behavioral genetics; biomedical facility; cognition; ethology; genetically modified animals; genotype; laboratory mouse; laboratory rat; phenotype

DESCRIPTION: The in vivo Behavioral Assessment Core will provide both service-oriented behavioral phenotyping and research staff training to investigators using rodents. One of the major goals of the Core will be the development and operation of a consolidated facility in which transgenic mice can reliably and consistently be tested using the most-accepted battery of behavioral tests, the SHIRPA (Rogers et al., 1997), along with expanded maze testing. The battery will include a primary behavioral screen, sensory and motor tests (including rotorod, spontaneous locomotor activity, walking coordination, etc.), an open field test for emotionality and exploratory activity and cognitive testing (including elevated plus-maze, Morris water maze, holeboard maze and eight-arm radial maze tasks). Most of the tasks will be automated, thereby greatly decreasing any potential investigator bias from the results. In most instances, the Initial phenotyping of a transgenic line will be carried out by the staff of the Core, who will be blinded to the genetic background of the mice. In those instances in which the investigator is interested in further characterizing the detailed behavior of a line of mice or a treatment, the staff and Director will train personnel from the investigator's lab to conduct more extensive studies, using the equipment of the Core. The Core will provide a similar assessment of rat behavior in apparatuses designed for these animals. For both rats and mice, the Core will, when deemed appropriate, test the animals using more sophisticated cognitive tasks for characterizing small differences between groups. For all animals tested, the Core will provide initial statistical analyses of resulting data in the reports to the investigators. The Core will also afford research personnel from the participating laboratories the opportunity to train in the use of these behavioral testing methods. This will particularly benefit investigators who require extended testing beyond the complexity offered in the standardized protocols of the Core. The Core will also assist investigators in the development of tools that are needed by them for a more detailed assessment of specific behaviors.

描述：体内行为评估核心将为使用啮齿动物的研究人员提供面向服务的行为表型分析和研究人员培训。核心的主要目标之一是开发和运营一个综合设施，在该设施中，可以使用最受接受的行为测试组 SHIRPA（Rogers 等人，1997 年）可靠且一致地对转基因小鼠进行测试扩大迷宫测试。该电池将包括初级行为屏幕、感觉和运动测试（包括旋转杆、自发运动活动、步行协调等）、情绪和探索活动的开放场地测试以及认知测试（包括高架十字迷宫、莫里斯水迷宫） , 洞板迷宫和八臂径向迷宫任务）。大多数任务将实现自动化，从而大大减少结果中潜在的研究者偏差。在大多数情况下，转基因品系的初始表型分析将由核心工作人员进行，他们对小鼠的遗传背景一无所知。在研究者有兴趣进一步表征小鼠品系或治疗的详细行为的情况下，工作人员和主任将培训研究者实验室的人员使用核心设备进行更广泛的研究。核心将在为这些动物设计的设备中对老鼠的行为进行类似的评估。对于大鼠和小鼠，核心将在被认为 适当的，使用更复杂的认知任务来测试动物，以表征组之间的微小差异。对于所有测试的动物，核心将在向研究人员提供的报告中提供结果数据的初步统计分析。核心还将为参与实验室的研究人员提供使用这些行为测试方法进行培训的机会。这将特别有利于那些需要超出核心标准化协议所提供的复杂性的扩展测试的研究人员。该核心还将协助研究人员开发他们所需的工具，以进行更详细的研究。 对具体行为的评估。