Priming the Memory Storage System

启动内存存储系统

• 批准号: 7113473
• 负责人: KEVIN A BOLDING
• 金额: $ 0.72万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7113473
• 关键词: antisense nucleic acid; behavior test; behavioral /social science research tag; behavioral genetics; conditioning; experience; fear; fluorescent in situ hybridization; gene environment interaction; genetic transcription; hippocampus; laboratory rat; memory; memory disorders; neural information processing; neurogenetics; neuropharmacology; oligonucleotides; predoctoral investigator; regulatory gene

DESCRIPTION (provided by applicant): Experiments are proposed to clarify the interaction between experience and transcription of immediate early genes in memory processing. Discoveries in this area could guide development of behavior and drug-based approaches to alleviation of the symptoms of hippocampal memory disorders. Aim 1 will determine the transcription-dependence of a rat's memory for context. The role of specific immediate early genes (lEGs) will be evaluated, as will the uptake, degradation, and dose-response of antisense oligodeoxynucleotides against the lEGs. Aim 2 will test the hypothesis that IEG transcription initiated by a context exposure could substitute for that normally induced after a separate, subsequent learning experience. Initial experiments will establish a behavioral priming effect in the context pre-exposure facilitation effect (CPFE) paradigm. We will then use the behavioral priming effect to block the effects of transcription inhibition in the CPFE. Finally, we will evaluate alternative hypotheses regarding the nature of the behavioral priming effect.

描述（由申请人提供）：提出实验来阐明记忆处理中的经验和立即早期基因转录之间的相互作用。这一领域的发现可以指导行为和基于药物的方法的发展，以减轻海马记忆障碍的症状。目标 1 将确定大鼠记忆的转录依赖性。将评估特定立即早期基因（IEG）的作用，以及针对 IEG 的反义寡脱氧核苷酸的摄取、降解和剂量反应。目标 2 将检验以下假设：由情境暴露引发的 IEG 转录可以替代通常在单独的后续学习经历后诱导的 IEG 转录。初步实验将在暴露前促进效应（CPFE）范式中建立行为启动效应。然后我们将利用行为启动效应来阻断 CPFE 中转录抑制的影响。最后，我们将评估有关行为启动效应性质的替代假设。