A muSI-RIAC instrument for multi-bioanalyte assays

用于多种生物分析物分析的 muSI-RIAC 仪器

• 批准号: 6690100
• 负责人: Louis Daniel Scampavia
• 金额: $ 10万
• 依托单位: FIA SOLUTIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690100
• 关键词: analytical chemistry; bioengineering /biomedical engineering; biomedical equipment development; fluid flow; fluorescence; immunoaffinity chromatography; luminescence; mass spectrometry; spectrometry

DESCRIPTION (provided by applicant): The aim of this grant is the development of a muSI-RIAC instrument that performs recycles immunoaffinity chromatography on a micro scale amount of sample such as urine, plasma or other biological based fluids. This project will synergistically combine the superior attributes of micro immunoaffinity chromatography with the high precision capabilities of micro sequential injection to create a very versatile and low cost instrumental platform for clinical diagnostics and biomedical analysis. The muSI-RIAC instrument will provide clinicians and researchers the ability to screen complex matrices for low abundance target analytes. This device holds great potential in many biomedical endeavors including biotechnology and drug discovery, immunology, and diagnostic clinical analysis. This device will use miniscule amounts of sample and quickly process it through multiple micro-affinity columns to isolate and then sequentially measure for the presence of targeted molecules. Benefits to the public health will impact both advancements in Research discovery as well as conventional Clinical diagnostics. Clinical situations where routine multi-analyte assays are required on pediatric, post-natal, or elderly patients may require excess sampling using conventional techniques. The serious discomfort or danger to the patients health and care can be greatly reduced through the muSI-RIAC instrument's ability to perform all needed assays with a single drop of fluid (e.g. blood, cerebral, spinal or lymphatic fluids). Research discovery is often encumbered by the need to perform multiple component analysis from highly valued yet small amounts of complex samples. For example, recombinant chemistries for drug discovery, archival blood samples, or cellular culture extracts. The ability to screen for low abundant target molecules, isolate, quantitative and retrieve such components would highly benefit the research communities ability to identify and study important bio-analyte interactions that serve to advance their respective fields.

描述（由申请人提供）：本次资助的目的是开发一种 muSI-RIAC 仪器，该仪器可对微量样品（如尿液、血浆或其他生物基液体）进行回收免疫亲和层析。该项目将协同地将微量免疫亲和层析的优越属性与微量连续注射的高精度能力结合起来，为临床诊断和生物医学分析创建一个非常通用且低成本的仪器平台。 muSI-RIAC 仪器将为临床医生和研究人员提供筛选复杂基质中低丰度目标分析物的能力。该设备在许多生物医学领域具有巨大潜力，包括生物技术和药物发现、免疫学和诊断临床分析。该设备将使用极少量的样品，并通过多个微亲和柱快速处理它，以分离并依次测量目标分子的存在。对公共健康的好处将影响研究发现和传统临床诊断的进步。 需要对儿科、产后或老年患者进行常规多分析物检测的临床情况可能需要使用传统技术进行过量采样。 muSI-RIAC 仪器能够用一滴液体（例如血液、脑液、脊髓液或淋巴液）执行所有需要的检测，从而大大减少对患者健康和护理造成的严重不适或危险。 研究发现常常因需要对高价值但少量的复杂样品进行多成分分析而受到阻碍。例如，用于药物发现的重组化学、档案血液样本或细胞培养物提取物。筛选低丰度目标分子、分离、定量和检索此类成分的能力将非常有利于研究界识别和研究重要生物分析物相互作用的能力，从而推动各自领域的发展。