Chemotherapy Drug Analyzer

化疗药物分析仪

• 批准号: 6444406
• 负责人: STUART FARQUHARSON
• 金额: $ 10万
• 依托单位: REAL-TIME ANALYZERS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-27 至 2003-09-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444406
• 关键词: Raman spectrometry; analytical chemistry; aniline; antineoplastics; benzoates; bioengineering /biomedical engineering; biomedical equipment development; camptothecin; capillary electrophoresis; carboplatin; clinical chemistry; cyclophosphamide; drug metabolism; fluorouracil; gel; p aminobenzoate; phenylacetates; portable biomedical equipment; silicates

DESCRIPTION (provided by applicant): The overall objective of the proposed program is to develop a bed-side analyzer capable of providing rapid identification and quantification of chemotherapy drugs and metabolites in serum and urine. The analyzer would allow optimizing effectiveness and minimizing adverse side-effects associated with chemotherapy drugs administered during cancer treatment. The analyzer will use capillary electrophoresis to separate drugs from body fluids with minimal sample preparation, Raman spectroscopy to identify the drugs, and surface-enhanced Raman spectroscopy (SERS) to increase sensitivity and allow quantification at physiologic concentrations. The Phase I program will demonstrate feasibility through three tasks with the following objectives: 1) develop the SER active sol-gel chemistry for selective adsorption of polar or nonpolar and positive or negative chemical groups; 2) test and refine chemical selectivity and SER activity of the sol-gels using p-aminobenzoic acid, phenyl acetylene, benzoic acid, aniline, camptothecin, carboplatin, cyclophosphamide, 5-fluorouracil, and 8-oxyguanine; and 3) test ability to selectively measure these chemicals and drugs with detection limits of <50 ng/ml and 5 ng/ml in simulated serum and urine samples, respectively. In Phase II a prototype bed-side analyzer will be engineered and head-to-head comparisons to existing technology will be performed.

描述（由申请人提供）：拟议的总体目标 该计划的目的是开发一种床边分析仪，能够快速识别和定量化疗药物和代谢物 血清和尿液。该分析仪将允许优化有效性和 最大限度地减少与化疗药物相关的不良副作用 在癌症治疗期间。该分析仪将使用毛细管电泳来 用最少的样品制备从体液中分离药物，拉曼 光谱鉴定药物，以及表面增强拉曼光谱 （SERS）以提高灵敏度并允许在生理学上进行定量 浓度。第一阶段计划将通过三个方面论证可行性 具有以下目标的任务：1）开发SER活性溶胶-凝胶 用于选择性吸附极性或非极性和正或的化学 负化学基团； 2) 测试和完善化学选择性和SER 使用对氨基苯甲酸、苯乙炔、苯甲酸的溶胶-凝胶的活性 酸、苯胺、喜树碱、卡铂、环磷酰胺、5-氟尿嘧啶和 8-氧鸟嘌呤； 3) 测试选择性测量这些化学物质的能力 模拟血清中检出限<50 ng/ml 和 5 ng/ml 的药物 分别是尿液样本。在第二阶段，原型床边分析仪将 与现有技术进行工程化和面对面的比较 执行。

项目成果

Surface-enhanced Raman spectral measurements of 5-fluorouracil in saliva.

唾液中 5-氟尿嘧啶的表面增强拉曼光谱测量。

• 发表时间: 2008-10-22
• 作者: Farquharson, Stuart;Gift, Alan;Shende, Chetan;Inscore, Frank;Ordway, Beth;Farquharson, Carl;Murren, John
• 通讯作者: Murren, John

Detection of drugs of abuse in saliva by surface-enhanced Raman spectroscopy (SERS).

通过表面增强拉曼光谱 (SERS) 检测唾液中的滥用药物。

• 发表时间: 2011-09
• 影响因子: 3.5
• 作者: Inscore, Frank;Shende, Chetan;Sengupta, Atanu;Huang, Hermes;Farquharson, Stuart
• 通讯作者: Farquharson, Stuart

Rapid detection and identification of overdose drugs in saliva by surface-enhanced Raman scattering using fused gold colloids.

使用熔融金胶体通过表面增强拉曼散射快速检测和识别唾液中的过量药物。

• 发表时间: 2011-07-13
• 影响因子: 5.4
• 作者: Farquharson, Stuart;Shende, Chetan;Sengupta, Atanu;Huang, Hermes;Inscore, Frank
• 通讯作者: Inscore, Frank