Durable Emitters for Nanospray Mass Spectrometry

用于纳喷雾质谱分析的耐用发射器

基本信息

批准号：
6802702
负责人：
Marie Moy
金额：
$ 25.11万
依托单位：
NANOGENESYS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-07-01 至 2005-09-29
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The long-term goal of this project is to develop miniaturized electrospray ionization (or nanospray) emitters that have enhanced durability and sensitivity versus those currently available. Nanospray mass spectrometry has improved detection limits, requires less sample volume, and uses lower flow rates (resulting in reduced consumption of analyte) than conventional electrospray ionization. However, commercial nanospray emitters which use thin gold films are readily destroyed by electrical discharge. An alternative to gold coatings for nanospray emitters employing conductive polyaniline has been developed at the University at Buffalo (UB). Polyaniline coatings exhibit high resistance to destruction by electrical discharge and retain the sensitivity improvements of nanospray over electrospray. The demonstrated superior durability of polyaniline-coated emitters to current commercial emitters should permit, for the first-time, routine coupling of capillary separations techniques to nanospray. The advantage of such a coupling is that it would permit highly sensitive detection and characterization of biological molecules and drugs directly from complex biological mixtures (e.g., cerebrospinal fluid, blood plasma, etc.). This powerful combination of analytical technologies will have important implications in detection of protein molecules indicative of disease states (e.g., hemoglobin, prealbumin). The immediate objective here is to demonstrate operational capillary electrophoresis and capillary liquid chromatography coupled to nanospray MS using polyaniline-coated emitters, and to produce commercially an integrated capillary column-nanospray emitter unit.

描述（由申请人提供）：该项目的长期目标是开发小型化电喷雾电离（或纳喷雾）发射器，与现有的发射器相比，其具有更高的耐用性和灵敏度。与传统电喷雾电离相比，纳喷雾质谱法提高了检测限，需要更少的样品体积，并且使用更低的流速（从而减少分析物的消耗）。然而，使用金薄膜的商业纳米喷雾发射器很容易被放电破坏。布法罗大学 (UB) 已开发出一种采用导电聚苯胺的纳米喷雾发射器金涂层的替代品。聚苯胺涂层表现出对放电破坏的高抵抗力，并保留了纳喷雾相对于电喷雾的灵敏度改进。聚苯胺涂层发射器与目前的商业发射器相比已证明具有优异的耐用性，这将首次允许毛细管分离技术与纳喷雾的常规耦合。这种耦合的优点在于，它可以直接从复杂的生物混合物（例如脑脊液、血浆等）中对生物分子和药物进行高度灵敏的检测和表征。这种分析技术的强大组合将对指示疾病状态的蛋白质分子（例如血红蛋白、前白蛋白）的检测产生重要影响。这里的直接目标是演示使用聚苯胺涂层发射器将毛细管电泳和毛细管液相色谱与纳喷雾质谱联用，并商业化生产集成毛细管柱-纳喷雾发射器单元。