Tandem Multi-modal Plate Readers for a High Throughput Screening platform

用于高通量筛选平台的串联多模式读板机

• 批准号: 10702003
• 负责人: Louis Daniel Scampavia
• 金额: $ 8.36万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10702003
• 关键词: Tandem; Multi; modal; Plate; Readers

PROJECT SUMMARY: The Scripps Research Molecular Screening Center (SRMSC) is requesting funds for the purchase of two microplate readers as a replacement for its current uHTS Perkin Elmer Viewlux which has reached end of life. The ViewLux has been the gold standard for high-speed microplate imaging; ideally suited for simultaneous plate-well imaging analysis where speed, reliability and high quality data is required. The integrated Viewlux reader on our HTS screening platform has served our needs admirably since its initial installment in 2005. However, after ~15-years of service, reliability has become a critical issue compounded by Perkin Elmer’s end of production (2017) and service support exacerbated by the discontinued manufacture of all parts/components. As of date, there is not a current reader on the market that equals the speed of the Viewlux, which is especially problematic with respect to ever increasing need to perform low light luminescence and BRET readouts. Not surprisingly, many comprehensive HTS screening centers (both Pharma & academic) must now rely on multiple readers to meet HTS automation throughput needs while maintaining high quality and reliable data. High Throughput Screening (HTS) has become an indispensable tool for probe development and drug discovery and the Scripps Research Molecular Screening Center (SRMSC) has been in the forefront in serving NIH investigators at Scripps and other academic institutes. Since establishing operations in 2005, we have screened over 300 targets to generate more than 100 million data points for our collaborators at Scripps and at other institutions. Over 122 scientific papers and numerous presentations demonstrate our ability to produce excellent research-driven data. Our screening results have led to the development of over 77 bioactive molecular probes as novel drug targets for biomedical studies that have successfully advanced as INDs for clinical trial testing and eventually NDA/FDA drugs. The tandem multi-modal plate readers will replace the aged ViewLux; allowing SRMSC to fulfill its current HTS screening obligations and continue to serve the NIH community at large with its comprehensive HTS drug discovery capabilities.

项目概要： 斯克里普斯研究分子筛选中心 (SRMSC) 正在申请资金购买 两台酶标仪作为其当前 uHTS Perkin Elmer Viewlux 的替代品，其已达到 ViewLux 已成为高速微孔板成像的黄金标准； 用于需要速度、可靠性和高质量数据的同步板孔成像分析。 我们的 HTS 筛选平台上的集成 Viewlux 阅读器自那时起就很好地满足了我们的需求 它于 2005 年首次安装。然而，经过约 15 年的使用，可靠性已成为一个关键问题 Perkin Elmer 的停产（2017 年）和服务支持加剧了这种情况 已停止生产所有零件/组件 截至目前，该产品上还没有当前的读者。 与 Viewlux 的速度相当的市场，这对于以往来说尤其成问题 执行低光发光和 BRET 读数的需求不断增加，这并不奇怪。 综合 HTS 筛选中心（制药和学术）现在必须依赖多个读者 满足 HTS 自动化吞吐量需求，同时保持高质量和可靠的数据。 通量筛选（HTS）已成为探针开发和药物开发不可或缺的工具 发现和斯克里普斯研究分子筛选中心（SRMSC）一直走在前列 自从在斯克里普斯和其他学术机构建立业务以来，一直为 NIH 研究人员提供服务。 2005年，我们筛选了300多个目标，为我们的研究生成了超过1亿个数据点 斯克里普斯和其他机构的合作者，发表了超过 122 篇科学论文和大量论文。 演示展示了我们产生出色的研究驱动数据的能力。 导致了超过 77 种生物活性分子探针的开发，作为新的药物靶标 生物医学研究已成功推进为临床试验测试的 IND，并最终 NDA/FDA 药物。串联多模式读板器将取代老化的 ViewLux； SRMSC 履行其当前的 HTS 筛查义务并继续为 NIH 社区服务 凭借其全面的高温超导药物发现能力。

项目成果

A new vulnerability to BET inhibition due to enhanced autophagy in BRCA2 deficient pancreatic cancer.

BRCA2 缺陷型胰腺癌中自噬增强导致 BET 抑制的新脆弱性。

• 发表时间: 2023-05-31
• 作者: Lee, EunJung;Archasappawat, Suyakarn;Ji, Keely;Pena, Jocelyn;Fernandez;Gangaraju, Ritika;Beesabathuni, Nitin Sai;Kim, Martin Jean;Tian, Qi;Shah, Priya;Scampavia, Louis;Spicer, Timothy;Hwang, Chang
• 通讯作者: Hwang, Chang

Protocol for high throughput 3D drug screening of patient derived melanoma and renal cell carcinoma.

对患者来源的黑色素瘤和肾细胞癌进行高通量 3D 药物筛选的方案。

• 发表时间: 2024-04
• 作者: Ortiz Jordan, Luis M;Vega, Virneliz Fernández;Shumate, Justin;Peles, Adam;Zeiger, Jordan;Scampavia, Louis;Spicer, Timothy P
• 通讯作者: Spicer, Timothy P

Identification of Potential Modulators of the RGS7/Gβ5/R7BP Complex.

RGS7/Gβ5/R7BP 复合体的潜在调节剂的鉴定。

• 发表时间: 2021-10
• 作者: Stoveken, Hannah M;Fernandez;Muntean, Brian S;Patil, Dipak N;Shumate, Justin;Bannister, Thomas D;Scampavia, Louis;Spicer, Timothy P;Martemyanov, Kirill A
• 通讯作者: Martemyanov, Kirill A

Identification of potent small molecule inhibitors of SARS-CoV-2 entry.

鉴定 SARS-CoV-2 进入的有效小分子抑制剂。

• 发表时间: 2022-01
• 作者: Mediouni, Sonia;Mou, Huihui;Otsuka, Yuka;Jablonski, Joseph Anthony;Adcock, Robert Scott;Batra, Lalit;Chung, Dong;Rood, Christopher;de Vera, Ian Mitchelle S;Rahaim Jr, Ronald;Ullah, Sultan;Yu, Xuerong;Getmanenko, Yulia A;Kennedy, Nicole M
• 通讯作者: Kennedy, Nicole M

HIGH-THROUGHPUT SCREENING AND DYNAMIC STUDIES OF SELECTED COMPOUNDS AGAINST SARS-COV-2

所选化合物抗 SARS-COV-2 的高通量筛选和动态研究

• 发表时间: 2024-09-14
• 作者: Oh;O. O.;O. Oh
• 通讯作者: O. Oh