Micromechanical Integrated DNA Analysis Technology

微机械集成DNA分析技术

• 批准号: 6801844
• 负责人: Mark A BURNS
• 金额: $ 112.18万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-23 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6801844
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; biotechnology; genetic techniques; nanotechnology

DESCRIPTION: (provided by applicant) The Program Project is focused on the development of integratable microfabricated DNA analysis components. This continuation proposal will develop nanoliter-scale DNA sample handling and analysis technologies for genotyping and for sequencing. The microfluidic, separation, and reaction components of genetic analysis are fabricated on silicon, glass, and/or polymer substrates. To optimize inexpensive design, testing, and production, the devices are made using photolithographic construction techniques. The proposal has three Program Project components, with the following goals: Project 1. Component Development and Refinement. Individual components are identified and are evaluated for suitability in an integrated system. Intensive engineering refinement occurs on a restricted number of component designs. Project 2. Advanced Component and Device Research. The basic engineering information required for advance optical detectors, microfluidic channels, novel fabrication methods, fluid control, surface interactions, and electrophoresis are obtained. Biological sample processing and DNA biochemistry procedures are developed for microscale integrated systems. Core Unit. Fabrication and Instrumentation Support. A central assembly and testing facility is maintained that can rapidly build and test prototypes and components for each of the projects. Experienced research engineers provide fabrication skills, knowledge, and training for the entire Program.

描述：（由申请人提供）该计划项目的重点是开发可集成的微型DNA分析组件。 该延续建议将开发用于基因分型和测序的纳米尺度DNA样品处理和分析技术。 遗传分析的微流体，分离和反应成分是在硅，玻璃和/或聚合物底物上制造的。 为了优化廉价的设计，测试和生产，设备是使用光刻构造技术进行的。 该提案具有三个计划项目组件，具有以下目标： 项目1。组件开发和改进。 识别单个组件并评估集成系统中的适用性。 密集的工程精炼发生在限制数量的组件设计上。 项目2。高级组件和设备研究。 获得了先进的光学探测器，微流体通道，新型制造方法，流体控制，表面相互作用和电泳所需的基本工程信息。 生物样品处理和DNA生物化学程序是针对微观集成系统开发的。 核心单元。 制造和仪器支持。 维持中央组装和测试设施，可以快速为每个项目构建和测试原型和组件。 经验丰富的研究工程师为整个计划提供制造技能，知识和培训。