Pancreatic Islet Imaging and Blood Flow

胰岛成像和血流

• 批准号: 6827742
• 负责人: JOHN C GORE
• 金额: $ 18.88万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6827742
• 关键词: SCID mouse; bioimaging /biomedical imaging; insulin dependent diabetes mellitus; kidney; liver; magnetic resonance imaging; microscopy; pancreas imaging /visualization; pancreatic islet transplantation; pancreatic islets

DESCRIPTION (provided by applicant): Pancreatic islet transplantation holds great promise for the treatment of type 1 diabetes; recent advances in islet isolation and immunosuppression have led to improved results. However, the inability to noninvasively assess islet mass or number, to quantify islet blood flow, or to image islets within the pancreas or after transplantation limit experimental approaches to increase or sustain islet mass after transplantation and hamper studies of islet function or pathology during the development of type 1 and type 2 diabetes. Using approaches involving optical imaging, biomedical engineering, and transplantation of murine and human islets, our multidisciplinary team proposes to develop and apply new imaging technology to assess islet blood flow and to image islets within the pancreas and after transplantation. The proposed studies bring two distinguished investigators from the in vivo imaging area (Gore/Lepage) to work with two investigators already working in the area of type 1 diabetes and islet biology (Powers/Piston). By bringing together scientists from different disciplines, the proposed studies apply rapidly expanding imaging technology from other areas of biology to assess islet blood flow and to image islets within the pancreas and after transplantation. Experimental approaches will include transplantation of islets into the liver or beneath the renal or hepatic capsule of an immunodeficient mouse model that allows long-term survival of murine islet grafts and human islet xenografts (NOD-SCID mice), real-time imaging of blood flow, and magnetic resonance imaging. Islet mass, survival, and function will be correlated with measurements of islet blood flow and with islet imaging and characterization by magnetic resonance. These studies should discover new information applicable to islet transplantation in humans and the examination of events that cannot be examined directly in patients who have undergone islet transplantation. These studies will lead to the ability to assess islet mass within the pancreas and after transplantation and such technology will be useful to a variety of diabetes investigators interested in type 1diabetes and islet transplantation.

描述（由申请人提供）： 胰岛移植对于治疗 1 型糖尿病具有广阔的前景；胰岛分离和免疫抑制方面的最新进展带来了更好的结果。然而，无法无创地评估胰岛质量或数量，量化胰岛血流量，或对胰腺内或移植后的胰岛进行成像，限制了移植后增加或维持胰岛质量的实验方法，并阻碍了发育过程中胰岛功能或病理学的研究1 型和 2 型糖尿病。我们的多学科团队建议使用光学成像、生物医学工程以及小鼠和人类胰岛移植等方法，开发和应用新的成像技术来评估胰岛血流并对胰腺内和移植后的胰岛进行成像。拟议的研究将来自体内成像领域 (Gore/Lepage) 的两名杰出研究人员与已经在 1 型糖尿病和胰岛生物学领域 (Powers/Piston) 领域工作的两名研究人员合作。通过汇集不同学科的科学家，拟议的研究应用来自其他生物学领域的快速扩展的成像技术来评估胰岛血流并对胰腺内和移植后的胰岛进行成像。实验方法包括将胰岛移植到免疫缺陷小鼠模型的肝脏或肾或肝被膜下方，从而使小鼠胰岛移植物和人胰岛异种移植物（NOD-SCID小鼠）长期存活，血流实时成像和磁共振成像。胰岛质量、存活率和功能将与胰岛血流量的测量以及通过磁共振进行的胰岛成像和表征相关。这些研究应该发现适用于人类胰岛移植的新信息，以及无法在接受胰岛移植的患者中直接检查的事件的检查。这些研究将能够评估胰腺内和移植后的胰岛质量，并且此类技术将对对 1 型糖尿病和胰岛移植感兴趣的各种糖尿病研究人员有用。