Pancreatic Islet Imaging and Blood Flow

胰岛成像和血流

• 批准号: 6827742
• 负责人: JOHN C GORE
• 金额: $ 18.88万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6827742
• 关键词: SCID mouse; bioimaging /biomedical imaging; insulin dependent diabetes mellitus; kidney; liver; magnetic resonance imaging; microscopy; pancreas imaging /visualization; pancreatic islet transplantation; pancreatic islets

DESCRIPTION (provided by applicant): Pancreatic islet transplantation holds great promise for the treatment of type 1 diabetes; recent advances in islet isolation and immunosuppression have led to improved results. However, the inability to noninvasively assess islet mass or number, to quantify islet blood flow, or to image islets within the pancreas or after transplantation limit experimental approaches to increase or sustain islet mass after transplantation and hamper studies of islet function or pathology during the development of type 1 and type 2 diabetes. Using approaches involving optical imaging, biomedical engineering, and transplantation of murine and human islets, our multidisciplinary team proposes to develop and apply new imaging technology to assess islet blood flow and to image islets within the pancreas and after transplantation. The proposed studies bring two distinguished investigators from the in vivo imaging area (Gore/Lepage) to work with two investigators already working in the area of type 1 diabetes and islet biology (Powers/Piston). By bringing together scientists from different disciplines, the proposed studies apply rapidly expanding imaging technology from other areas of biology to assess islet blood flow and to image islets within the pancreas and after transplantation. Experimental approaches will include transplantation of islets into the liver or beneath the renal or hepatic capsule of an immunodeficient mouse model that allows long-term survival of murine islet grafts and human islet xenografts (NOD-SCID mice), real-time imaging of blood flow, and magnetic resonance imaging. Islet mass, survival, and function will be correlated with measurements of islet blood flow and with islet imaging and characterization by magnetic resonance. These studies should discover new information applicable to islet transplantation in humans and the examination of events that cannot be examined directly in patients who have undergone islet transplantation. These studies will lead to the ability to assess islet mass within the pancreas and after transplantation and such technology will be useful to a variety of diabetes investigators interested in type 1diabetes and islet transplantation.

描述（由申请人提供）： 胰岛移植对1型糖尿病的治疗有很大的希望。胰岛分离和免疫抑制的最新进展导致了结果的改善。但是，无法无创地评估胰岛质量或数量，量化胰岛流动，或在胰腺内的图像胰岛或在移植极限之后的实验方法，以增加或维持对1型和2型糖尿病期间胰岛功能或病理的胰岛功能或病理后增加胰岛质量的实验方法。使用涉及光学成像，生物医学工程以及鼠和人类胰岛移植的方法，我们的多学科团队建议开发和应用新的成像技术来评估胰岛流动，并在胰腺和移植后图像图像胰岛。拟议的研究使来自体内成像区域（Gore/Lepage）的两名杰出研究者与已经在1型糖尿病和胰岛生物学领域（Powers/Piston）领域工作的研究者合作。通过将来自不同学科的科学家汇集在一起​​，提出的研究将迅速扩展的成像技术从其他生物学领域迅速扩展，以评估胰岛流量和胰腺内和移植后的胰岛图像。实验方法将包括将胰岛移植到肝脏中，或在免疫缺陷小鼠模型的肾脏或肝囊下移植，该模型允许鼠胰岛移植物和人类胰岛异种移植物（NOD-SCID小鼠）长期存活，血液流动的实时成像以及磁性共鸣成像。胰岛质量，生存和功能将与胰岛流动的测量以及胰岛成像和通过磁共振表征相关。这些研究应发现适用于人类胰岛移植的新信息，以及对经过胰岛移植的患者无法直接检查的事件的检查。这些研究将导致能够评估胰腺内的胰岛质量，并且在移植后，此类技术将对对1糖类类型和胰岛移植感兴趣的各种糖尿病研究人员有用。