Measurement Of Cytokines In Cervical Fluids

宫颈液中细胞因子的测量

• 批准号: 6837023
• 负责人: Terry M. Phillips
• 金额: --
• 依托单位: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6837023
• 关键词: cervical /vaginal smear; cervix neoplasms; cytodiagnosis; cytokine; diagnosis design /evaluation; diagnostic tests; disease /disorder proneness /risk; genital secretion; gynecology; human papillomavirus; human tissue; immunoaffinity chromatography; neoplasm /cancer diagnosis; viral carcinogenesis; virus related neoplasm /cancer; women's health

The relationship between Herpes viruses and neoplasia has been quite well documented although the role played by host factors in this relationship is still unknown. The prime objective of this project to determine whether multi-analyte cytokine profiles measured in cervical specimens, from women infected with oncological types of Human Papillomavirus (HPV), can predict risk of subsequent progression to high-grade cervical intraepithelial neoplasia (CIN). Using a technique called recycling immunoaffinity chromatography (RIC), examination of cytokine profiles in cervical fluid will be performed to determine which cytokines and other factors appear to be elevated or activated during the neoplastic process. 30 analyte panels will be employed to compare cytokine profiles in samples from women with high-grade CIN to samples from women with oncogenic HPV infection and normal controls. From such studies it is hoped to determine which cytokines or other factors are candidates for predicting neoplastic progression. Once this has been established these markers will be used in a prospective study of HPV and CIN natural history. The initial studies demonstrated the efficacy of the RIC system and have shown that cytokine panels are useful in determining the different patient groups. Further studies have demonstrated the RIC system to be reliable and reproducible. Work is now progressing to apply this analytical system to epidemiological field studies.

尽管宿主因素在这种关系中所起的作用仍然未知，但疱疹病毒与肿瘤之间的关系已得到充分记录。该项目的主要目的是确定在宫颈标本中测得的多分析物细胞因子谱，从感染肿瘤学类型的人乳头瘤病毒（HPV）的妇女中是否可以预测随后发展为高级宫颈内上皮内肿瘤（CIN）的风险。使用称为回收免疫亲和色谱的技术，将进行宫颈液中细胞因子谱的检查，以确定在肿瘤过程中似乎升高或激活的细胞因子和其他因素。将使用30个分析板来比较来自具有高级CIN的女性的样品与来自致癌性HPV感染和正常对照的女性的样品的样品。从这些研究中，希望确定哪些细胞因子或其他因素是预测肿瘤进展的候选者。建立了这些标记后，将使用这些标记在HPV和CIN自然史的前瞻性研究中。最初的研究证明了RIC系统的功效，并表明细胞因子面板在确定不同的患者组方面很有用。进一步的研究表明，RIC系统是可靠和可重复的。现在正在进行工作，将这种分析系统应用于流行病学现场研究。