Core--Cell Culture and Genetic Engineering

核心--细胞培养与基因工程

• 批准号: 6797586
• 负责人: JAYANTA ROY-CHOWDHURY
• 金额: $ 15.43万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6797586
• 关键词: Adenoviridae; Baculoviridae; Retroviridae; biomedical equipment resource; biomedical facility; cell line; fluorescence microscopy; genetic manipulation; in situ hybridization; liver cells; phase contrast microscopy; tissue /cell culture; tissue /cell preparation; tissue resource /registry; transfection; transfection /expression vector

Objectives of the Cell Culture and Genetic Engineering Core are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (ii) assistance in developing recombinant viral vectors, (based on retroviruses, adenoviruses and baculoviruses) for use in their specific research, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth; (v) equipment and instructions for use in isolation and culturing of various cell types derived from the liver; and (vi) equipment and assistance in microscopy of cultured cells following genetic or other manipulations.

细胞培养和基因工程核心的目标是为肝脏研究中心提供 研究人员：（i）始终高质量的孤立肝细胞，（ii）开发的帮助 重组病毒载体（基于逆转录病毒，腺病毒和杆状病毒）用于其特定研究，（iii）细胞系的特征良好的冷冻库存，（IV）专用培养基，激素和补充剂，以及基质成分以及基质成分以用于最佳细胞生长； （v）用于隔离和培养从肝脏衍生的各种细胞类型的设备和说明； （vi）遗传或其他操纵后培养细胞显微镜的设备和辅助。