Sperm recognition genes

精子识别基因

• 批准号: 6643424
• 负责人: JULIE A ALIPAZ
• 金额: $ 4.16万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6643424
• 关键词: animal population genetics; cell cycle; cell line; complementary DNA; developmental genetics; differential display technique; egg /ovum; embryogenesis; fertilization; gene expression; genome; messenger RNA; nucleic acid sequence; polymerase chain reaction; receptor binding; sea urchins; species difference; sperm

DESCRIPTION: (Scanned from the applicant?s abstract) The successful fusion of sperm and egg is the first and most critical step in an organism?s development. Understanding how and when fertilization successful takes place is critical to understanding population and species divergence, but the mechanisms of cell formation and division. The model organism with which to study gametic interactions is the free spawning sea urchin Echinometra, where sperm-egg recognition is the only barrier against undesirable or incompatible fertilizations. The protein that in part mediates the species-specific binding of sperm to the egg surface, bindin, is well characterized. The corresponding receptor of the egg surface however, has yet to be identified. I propose here an attempt to identify this and other female specific genes that are expressed only in the germ line tissues via RNA differential display. Female specific transcripts associated with the bindin gene (that is male specific are) perfect candidates for the unknown egg surface receptor. All female specific transcripts will be explored for their possible involvement in gametic interactions and ultimately species divergence.

描述：（从申请人的摘要中扫描）成功融合 精子和卵子是有机体发育的第一步也是最关键的一步。 了解受精成功的方式和时间对于 了解种群和物种差异，但细胞的机制 形成和分裂。研究配子的模型生物 相互作用是自由产卵的海胆 Echinometra，其中精子与卵子 认可是防止不良或不相容的唯一障碍 施肥。部分介导物种特异性结合的蛋白质 精子与卵子表面的结合蛋白（bindin）已得到很好的表征。相应的 然而，鸡蛋表面的受体尚未确定。我在这里提议 试图识别这个基因和其他表达的女性特定基因 仅在种系组织中通过 RNA 差异显示。女性专用 与结合基因（即男性特有）相关的转录本是完美的 未知鸡蛋表面受体的候选者。所有女性专用 将探索转录本是否可能参与配子 相互作用并最终导致物种分化。