Intra-and Inter-Species Communication in Bacteria

细菌的种内和种间通讯

• 批准号: 6931936
• 负责人: BONNIE L BASSLER
• 金额: $ 39.35万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6931936
• 关键词: Vibrio; bacterial genetics; bacterial proteins; biological signal transduction; cell cell interaction; functional /structural genomics; fusion gene; inhibitor /antagonist; microorganism interaction; molecular cloning; protein protein interaction; reporter genes; stimulant /agonist

DESCRIPTION (provided by applicant): The long-term goal of this research is to explore the molecular mechanisms that bacteria use for intra-and inter-species cell-cell communication. The objective of this proposal is to identify and study the model Vibrio harveyi signal transduction pathway that links detection of extracellular quorum sensing signal molecules termed Al-I and Al-2 to target gene responses. Definition of the genes, proteins, protein-protein interactions, protein modifications and signaling molecules involved in this cell-cell communication system will lead to a molecular understanding of how signals are detected and how this information is integrated, processed, and transduced to control behavior on a community scale. Analyzing the V. harveyi quorum sensing signaling circuit should be valuable for understanding how bacteria perceive and react to their environment, how they respond to temporally coincident cues, and how they communicate with one another to coordinate behavior. Since the regulatory process is complex and involves both intra-species, inter-species, intra-cellular and inter-cellular signal transmission, interesting new mechanisms should be revealed

描述（由申请人提供）：本研究的长期目标是探索细菌用于种内和种间细胞间通讯的分子机制。本提案的目的是识别和研究哈维弧菌信号转导通路模型，该通路将细胞外群体感应信号分子（称为 Al-1 和 Al-2）的检测与靶基因反应联系起来。对细胞间通讯系统中涉及的基因、蛋白质、蛋白质-蛋白质相互作用、蛋白质修饰和信号分子的定义将导致对如何检测信号以及如何整合、处理和转导这些信息以控制行为的分子理解在社区规模上。分析哈维氏弧菌群体感应信号回路对于了解细菌如何感知环境并对其做出反应、它们如何响应时间上一致的线索以及它们如何相互沟通以协调行为具有重要意义。由于调控过程很复杂，涉及种内、种间、细胞内和细胞间信号传递，因此应该揭示有趣的新机制