ZEISS AXIOVERT 200 AUTOMATED 4-D CELL OBSERVER SYSTEM

ZEISS AXIOVERT 200 自动化 4D 细胞观察系统

• 批准号: 6581497
• 负责人: Steven D Leach
• 金额: $ 12.55万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581497
• 关键词: biomedical equipment purchase; charge coupled device camera; fluorescence microscopy; intravital microscopy; light microscopy

DESCRIPTION (provided by applicant): Recent advances in automated robotics, high-resolution digital imaging and image deconvolution software now allow real-time documentation of dynamic processes in living cells and tissues. To take advantage of this progress, we have assembled an accomplished group of senior, mid-career, and junior investigators who wish to apply this emerging technology to their NIH-funded research projects. Together, these six Johns Hopkins faculty members serve as principle investigators on ten different NIH grants including nine R01 grants and one T32 training grant, together accounting for 51 years of cumulative uninterrupted NIH funding. These research projects are funded by six Jdifferent NIH institutes, reflecting a a wide array of research areas, including epithelial and hematopoietic stem cell biology, development biology, developmental neurobiology, macrophage function, and tumor biology. In order to apply recent advances in real time imaging to these projects, the investigators seek to purchase a fully integrated state-of-the-art imaging system comprised of a Zeiss Axiovert-200M motorized inverted fluorescent microscope, a Zeiss AxioCam high-resolution digital camera, a temperature / humidity / CO2-controlled stage chamber for maintenance of viable cell and tissue explants, and sophisticated Zeiss Axioversion software with 3D image deconvolution capability. Together, this Cell Observer System allows dynamic cellular processes to be observed at high resolution using multiple fluorescent channels over extended periods of time, generating new perspectives regarding integrated biologic processes in living tissues. This proposal outlines the scientific rational for use of this sophisticated imaging system, and further describes available institutional support as well as administrative and laboratory resources designed to promote optimal use of this shared instrumentation.

描述（由申请人提供）：自动化机器人技术，高分辨率数字成像和图像反卷积软件的最新进展现在允许实时记录活细胞和组织中的动态过程。为了利用这一进展，我们组建了一群成熟的高级，职业和初级调查员，他们希望将这种新兴技术应用于其NIH资助的研究项目。这六个约翰·霍普金斯（Johns Hopkins）的教职员工一起担任了十个不同的NIH赠款的主要调查员，其中包括9份R01赠款和一项T32培训赠款，共同考虑了51年累计不间断的NIH资金。这些研究项目由六个Jdiverent NIH机构资助，反映了各种研究领域，包括上皮和造血干细胞生物学，发育生物学，发育神经生物学，巨噬细胞功能和肿瘤生物学。为了将最新进展应用于这些项目，研究者试图购买由Zeiss Axiovert 200M制动的完全集成的最先进的成像系统，该系统机动倒荧光显微镜，Zeiss AxioCam高分辨率高分辨率数字数字相机，用于维护可行的单元格和组织外植体的温度 /湿度 /二氧化碳控制的阶段室以及具有3D图像反卷积能力的复杂Zeiss Axioversion软件。总之，该细胞观察者系统允许在长时间使用多个荧光通道在高分辨率下观察动态细胞过程，从而产生有关活组织中综合生物学过程的新观点。该提案概述了使用此复杂成像系统的科学合理性，并进一步描述了可用的机构支持以及旨在促进这种共享仪器最佳使用的行政和实验室资源。