ACQUISITION OF A DIGITAL MICROSCOPY RESOURCE

数字显微镜资源的获取

• 批准号: 2488361
• 负责人: STEVE A KAY
• 金额: $ 26.41万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2488361
• 关键词: biomedical equipment; biomedical equipment purchase; cell biology; charge coupled device camera; computer program /software; confocal scanning microscopy; digital imaging; fluorescence microscopy; image processing; imaging /visualization /scanning

Progress in cutting-edge biomedical studies has become increasingly dependent upon the technology required to measure biological process as they occur in living organisms, tissues, and individual cells. In recent years, increasing numbers of probes have been developed that can detect cellular process in live cells as have the technologies and tools to monitor these activities. Research groups at The Scripps Research Institute have initiated activities to image processed in live cells in several areas. Using green fluorescent protein (GFP) to track intra- and intercellular dynamics of many proteins, including movement of centromeric proteins during mitosis, targeting of proteins to the nuclear envelope, virus movement, neuronal cytoskeleton dynamics and monitoring expression of clock proteins. Others are developing and applying novel fluorescent analogs to indicators for studies in live cell function. Our goal is to establish at TSRI a unique facility with equipment and software that will enable us to study live cells in real-time with the capacity to capture light in optical sections, to deconvolve the capture light, carry out ratio image, and to reconstruct patterns of fluorescence through cells. The system will perform bright field and differential interference contrast as well as low-light fluorescence and luminescence imaging of animal and plant cells and tissues. We propose to acquire a Deltavision digital microscopy system that will emphasize the following features: optimized optical paths to reduce light loss, several types of stage chambers for the maintenance of living specimens during image acquisition, a high resolution/high readout rate cooled CCD camera, optical sectioning capabilities and proven deconvolution software for assembling 3D images, and silicon graphics workstations for rapid convenient image acquisition and processing. While each of the participants has existing fluorescence microscopy capabilities within their labs, this equipment will enable areas of research far beyond those currently available at this Institute. Furthermore, this Digital Microscopy Resource system for live cell processes will be included within the physical facility that also contains confocal and electron microscopes, and will serve a large diverse biology community at TSRI. It will also initiate opportunities for interdisciplinary collaboration with other well established TSRI programs in computational biology, imaging science, and biological structure through member participation on the Advisory board that will oversee the use of the instrument.

前沿生物医学研究的进展日益增多 取决于测量生物过程所需的技术 它们存在于活的有机体、组织和单个细胞中。近来 多年来，越来越多的探针被开发出来，可以检测 活细胞中的细胞过程，拥有技术和工具 监控这些活动。斯克里普斯研究中心的研究小组 研究所发起了在活细胞中处理图像的活动 几个领域。使用绿色荧光蛋白 (GFP) 追踪内部和外部 许多蛋白质的细胞间动力学，包括着丝粒的运动 有丝分裂期间的蛋白质，将蛋白质靶向核膜， 病毒运动、神经细胞骨架动力学和表达监测 时钟蛋白。其他人正在开发和应用新型荧光 类似于活细胞功能研究的指标。我们的目标是 在 TSRI 建立一个独特的设施，配备设备和软件， 使我们能够实时研究活细胞并具有捕获能力 光在光学部分，去卷积捕获光，进行 比率图像，并重建穿过细胞的荧光图案。 系统将进行明场和微分干涉 对比度以及低光荧光和发光成像 动物和植物细胞和组织。我们建议收购 Deltavision 数字显微系统将强调以下功能： 优化光路以减少光损失，多种类型的平台 用于在图像采集过程中维护活体标本的室， 高分辨率/高读出率冷却 CCD 相机、光学切片 用于组装 3D 图像的功能和经过验证的反卷积软件， 和硅图形工作站，可快速方便地采集图像 和加工。虽然每个参与者都有现有的荧光 实验室内的显微镜功能，该设备将使 其研究领域远远超出了该研究所目前的研究范围。 此外，该活细胞数字显微镜资源系统 流程将包含在物理设施内，该设施还包含 共焦显微镜和电子显微镜，并将服务于大量多样化的生物学 TSRI 社区。它还将为 与其他成熟的 TSRI 项目进行跨学科合作 计算生物学、成像科学和生物结构 通过成员参与顾问委员会，该委员会将监督 仪器的使用。