CORE--ANTIBODY/RADIOIMMUNOASSAY

核心--抗体/放射免疫分析

• 批准号: 6410314
• 负责人: JOHN H WALSH
• 金额: $ 14.67万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6410314
• 关键词: biomedical facility; gastrointestinal system; immunocytochemistry; immunofluorescence technique; immunologic assay /test; monoclonal antibody; radioimmunoassay; radiotracer; technology /technique development; tissue /cell culture

DESCRIPTION (Adapted from the application) The objectives of this core are to develop polyclonal and monoclonal antibodies useful for radioimmunoassay, for immunohistochemistry, and for immunoneutralization and to assist center members in the use of these antibodies. A second objective is to prepare radiolabeled and fluorescent peptides for use in radioimmunoassays, receptor binding, and receptor internalization studies. The core produces the antibody, develops the required assays, and provides the kits that are used by members at the center according to their own experiments. The core also provides teaching services for techniques and procedures including Western blot analysis, radio-iodination, ELISA, antibody production, antibody purification and Fc fragment production, RIA procedures, and fluorescence conjugations. These services will be used by CURE investigators, technicians, and postdoctoral fellows. In addition, the core will perform non-routine RIAs upon request. Antisera and monoclonal antibodies produced by the core are tabulated and are made available to center investigators. These available antibodies are listed on the CURE Web page and are made available to investigators outside the center. The space for this core consists of twin 500 square feet laboratories in the UCLA Division of Digestive Diseases adjacent to the office of Dr. Walsh. The laboratory is fully equipped for its current functions. Previously produced antibodies are readily distributed upon request after approval of the Director. Request for preparation of new antibodies requires consultation with the Director to determine the feasibility of the project and the most practical approach. A large number of center investigators, as well as scientists worldwide, have been provided core services, and many papers have resulted from interactions with this core. Between 1993 and June 1998, eighty-seven papers from the center have resulted from interactions with this core. During the period, there are 44 additional papers published by investigators who were not members of the CURE but who utilized the core services.

描述（改编自应用程序）该核心的目标是 开发用于放射免疫分析的多克隆和单克隆抗体， 免疫组织化学，用于免疫中和并协助中心成员 在使用这些抗体时。第二个目标是制备放射性标记的 和荧光肽用于放射免疫测定、受体结合和 受体内化研究。核心产生抗体，开发 所需的检测，并提供中心成员使用的试剂盒 根据他们自己的实验。核心还提供教学服务 包括蛋白质印迹分析在内的技术和程序， 放射性碘、ELISA、抗体生产、抗体纯化和 Fc 片段产生、RIA 程序和荧光缀合。这些 服务将由 CURE 研究人员、技术人员和博士后使用 各位。此外，核心还将根据请求执行非常规 RIA。 核心产生的抗血清和单克隆抗体被制成表格并 提供给中心调查人员。列出了这些可用的抗体 在 CURE 网页上，可供外部研究人员使用 中心。 该核心的空间由两个 500 平方英尺的实验室组成，位于 加州大学洛杉矶分校消化疾病科毗邻沃尔什医生的办公室。这 实验室设备齐全，可满足其当前的功能。以前生产过的 经主任批准后，可根据要求随时分发抗体。 请求制备新抗体需要咨询 总监确定项目的可行性和最实用的 方法。大量的中心研究人员以及科学家 在全球范围内提供了核心服务，并发表了许多论文 与这个核心的相互作用。 1993年至1998年6月期间，发表了87篇论文 来自中心的结果是与该核心相互作用的结果。期间 在此期间，还有 44 篇由研究人员发表的论文，但这些论文并未被 CURE 的成员，但使用了核心服务。