CORE--MOLECULAR IMAGING

核心--分子影像

• 批准号: 6316581
• 负责人: John J Lemasters
• 金额: $ 16.67万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316581
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; fluorescence microscopy; gastrointestinal disorder; gastrointestinal system; histology; immunocytochemistry; in situ hybridization; intravital microscopy

DESCRIPTION (Adapted from the application) The Molecular Imaging Core provides an extremely useful array of facilities and services. These include the following components. 1. Confocal Microscopy Component, including facilities for 1) high-resolution 3-dimensional imaging of parameter-sensitive fluorophores in living cells; 2) intravital microscopy of the livers of anesthetized rats; 3) high-resolution imaging of tissue sections; 4) a fluorescence plate reader for monitoring outcomes in multi-well plates, and 5) an experienced confocal microscopist to provide training and orientation to CGIBD investigators. 2. Histology/ Pathology Component, including basic histological and immunohistochemical services for preparation of frozen and paraffin sections for histology, immunocytochemistry, and in situ hybridization. These services will support the histological analysis of liver, pancreas, intestine and any other tissues under scrutiny by center investigators. This component will also assist in carrying out immunohistochemistry studies as required for the various center projects. 3. In Situ Hybridization Component to identify expression of specific mRNA species within tissue sections and to provide training and assistance to investigators in the preparation of cRNA and cDNA probes, hybridization of these probes to tissue sections and tissue localization of hybrids by peroxidase and immunofluorescent cytochemistry. 4. Digital Darkroom Component for analysis of gels and micrographs and preparation of high quality prints, slides, and figures for use in publications, reports and oral presentations. 5. Molecular Biology Consultation Component in which core specialists will provide consultation and training to CGIBD members in molecular biology techniques, including Western and Northern blotting, electrophoretic mobility shift assays, and differential display techniques. As needed, consultants will direct investigators to other cores on the campus of the UNC for DNA and protein sequencing, polypeptide and oligonucleotide synthesis, and transgenic animals.

描述（改编自应用程序）分子成像核心提供 一系列极其有用的设施和服务。这些包括 以下组件。 1. 共焦显微镜组件，包括设施 1）参数敏感的高分辨率3维成像 活细胞中的荧光团； 2) 肝脏活体显微镜检查 麻醉大鼠； 3）组织切片高分辨率成像； 4）一个 用于监测多孔板结果的荧光读板器，以及 5) 经验丰富的共焦显微镜师为您提供培训和指导 CGIBD 调查员。 2. 组织学/病理学部分，包括基础 组织学和免疫组织化学服务，用于制备冷冻和 用于组织学、免疫细胞化学和原位的石蜡切片 杂交。这些服务将支持肝脏的组织学分析， 中心检查的胰腺、肠和任何其他组织 调查人员。该组件还将协助执行 各个中心项目所需的免疫组织化学研究。 3. 在 原位杂交组件可鉴定特定 mRNA 种类的表达 在组织切片中并为研究人员提供培训和帮助 在制备 cRNA 和 cDNA 探针时，这些探针与 通过过氧化物酶和杂交体的组织切片和组织定位 免疫荧光细胞化学。 4. 用于分析的数字暗室组件 凝胶和显微照片以及高质量印刷品、幻灯片和的制备 用于出版物、报告和口头演示的数字。 5. 分子 生物学咨询部分，核心专家将提供 为 CGIBD 成员提供分子生物学技术方面的咨询和培训， 包括蛋白质印迹和北方印迹、电泳迁移率变化分析、 和差分显示技术。根据需要，顾问将指导 北卡罗来纳大学校园内其他核心的 DNA 和蛋白质研究人员 测序、多肽和寡核苷酸合成以及转基因动物。