High Productivity Eukaryotic Cell Culture Technology

高产真核细胞培养技术

• 批准号: 6833399
• 负责人: Robin A Felder
• 金额: $ 9.96万
• 依托单位: MEDICAL ROBOTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2006-06-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833399
• 关键词: bladder neoplasm; cell morphology; cell proliferation; eukaryote; glass; method development; neoplastic cell; particle; prostate neoplasms; tissue /cell culture

DESCRIPTION (provided by applicant): The goals of this project are to develop a method to increase eukaryotic cell production and cell quality for research and drug discovery. We have preliminary data that demonstrates that we have developed a neutral buoyancy hydrogel based microcarrier process that can dramatically increase the density, viability, and diversity of cells available to researchers while dealing with their limited space and increase productivity needs. Our novel microcarrier based cell culture process allows anchorage dependent cells to be transferred from the growth media directly into an analytical process without the need to use trypsin (or other means) to remove cells from their anchorage surface. Our microcarriers are optically clear and do not exhibit any autofluorescence, thus they are ideal for light based microscopy and in vitro fluorescence studies. We have demonstrated that the density of the microcarriers can be manipulated though the addition of glass bubbles and paramagnetic particles to allow suspension growth without the use of impellers. Non-contact magnetic manipulation permits the entire cell growth process to be used with laboratory automation. Given our preliminary data, this proposal is centered on three specific aims: 1. We will refine our preliminary method for producing neutral buoyancy microcarriers that are optimized for the growth of a variety of prostate cancer and bladder cancer cells in order to make them microcarriers available to cancer center research scientists for user feedback. 2. We will culture cells on our novel microcarriers in order to measure cell viability, density, and morphology. 3. We will develop a conceptual framework for the design of an automated cell culture system based on our many years of experience in laboratory automation and cell culture. Our novel neutral density microcarriers will improve the productivity, quality, and availability of eukaryotic cells for research scientists.

描述（由申请人提供）：该项目的目标是开发一种方法来提高真核细胞的生产和细胞质量以进行研究和药物发现。我们拥有初步数据，这些数据表明我们已经开发了基于中性的浮凝胶微载体工艺，该过程可以大大提高研究人员可用的细胞的密度，生存力和多样性，同时处理其有限的空间并增加了生产力需求。我们新型的基于微载体的细胞培养过程允许将锚定依赖性细胞直接从生长培养基转移到分析过程中，而无需使用胰蛋白酶（或其他方式）从其锚固表面去除细胞。我们的微载体在光学上是清晰的，并且没有表现出任何自动荧光，因此它们非常适合轻度显微镜和体外荧光研究。我们已经证明，可以通过添加玻璃气泡和顺磁性颗粒来操纵微载体的密度，以允许无需使用叶轮而悬浮生长。非接触式磁操纵允许与实验室自动化一起使用的整个细胞生长过程。 鉴于我们的初步数据，该提案以三个具体目的为中心： 1。我们将完善我们的初步方法，用于生产中性浮力微载体，这些微载体对各种前列腺癌和膀胱癌细胞的生长进行了优化，以便使其可为癌症中心研究科学家使用的微载体提供用户反馈。 2。我们将在新型的微载体上培养细胞，以测量细胞活力，密度和形态。 3。我们将根据我们在实验室自动化和细胞培养方面的多年经验来为自动细胞培养系统设计设计一个概念框架。 我们的新型中性密度微载体将提高真核细胞为研究科学家的生产率，质量和可用性。