梯度强/超强静磁场对细胞有丝分裂纺锤体取向和形态的影响及机制研究

With the development of high-field magnetic resonance imaging and other technologies, the biological effects and safety of high static magnetic fields have attracted people's attentions. Although it has been reported that gradient high magnetic fields affected the viability of human and mouse bone cells, and could also suspend small animals such as frog, the effects on the mitotic spindle of human cells are still unclear. The previous study by the applicant found that the 27 T ultra-high uniform static magnetic field affected the orientation and morphology of the mitotic spindle of human cells, but the effects in the gradient high static magnetic fields are still unclear. The applicant intends to study 15 different cell lines, including tumor and non-tumor cells, and use the "water-cooled magnet #4" in High Magnetic Field Laboratory and the cell culture device built by the applicant to further study the effects of gradient high/ultra-high static magnetic field on spindle orientation and morphology, cell proliferation, cycle and apoptosis, as well as spindle localization and morphology related proteins changes, and the force/torque acted on the spindle in the gradient high/ultra-high static magnetic field will be analyzed and calculated. Finally, the biological and physical mechanism of its orientation and shape changes will be found. The implementation of this project is expected to provide a new technique for the study of mitotic spindles and provide a basis for the safety of high-field MRI.

随着高场磁共振成像等技术的发展，强磁场的生物学效应及安全性问题引起人们的关注。尽管梯度强静磁场会影响人和鼠骨细胞的活力，还能使青蛙等小动物悬浮，但对人类细胞有丝分裂纺锤体的影响还不明晰。申请人前期研究发现27T超强均匀静磁场会影响人类细胞有丝分裂纺锤体的取向和形态，但在梯度强/超强静磁场中的效应还不清楚。本项目拟以15种不同组织来源和遗传背景的肿瘤和非肿瘤细胞为研究对象，利用国家稳态强磁场实验装置WM4号水冷磁体和申请人搭建的细胞培养装置，进一步研究梯度强/超强静磁场对细胞有丝分裂纺锤体取向和形态的影响，对细胞增殖、周期和凋亡等方面的影响，并检测曝磁后纺锤体定位及形态相关蛋白的变化情况，计算分析纺锤体在梯度强/超强静磁场中受磁力或磁力矩的情况，最终找到其取向和形态变化的生物和物理机制。本项目的实施预期将为细胞有丝分裂纺锤体的研究提供一种新的技术手段，同时为高场MRI的安全性问题提供依据。