Core--Inhalation /transgenic mouse

核心--吸入/转基因小鼠

基本信息

批准号：
6901795
负责人：
DAVID R. HEMENWAY
金额：
$ 25.52万
依托单位：
UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-06-04 至 2006-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6901795
关键词：
animal colony biomedical facility genetically modified animals laboratory mouse pulmonary fibrosis /granuloma

项目摘要

The Specific Aims of the Inhalation Core are to provide housing of mice prior to inhalation experiments in a pathogen-free environment and controlled exposures to asbestos and ovalbumin (OVA). The objective of the Transgenic Mouse Core is to provide transgenic mice to the members of the Program Project. The generation, maintenance and screening of new and previously generated conventional transgenic mice will be centralized in this Core. In addition, this Core aims to develop a new experimental approach that involves stable integration of bacterial artificial chromosomes (BACs) in the mouse genome. The Specific Aims of this Core are: i. To provide a Core service for the generation of new transgenic mice (F2 C3H x C57Bl/6) expressing specific transgenes in pulmonary epithelial cells. The new transgenic mice that are proposed in this Program Project will be generated in the University of Vermont Transgenic Mouse Core, directed by Dr. Rincon. ii. To centralize the backcross process required to obtain each of the new and previously generated transgenic mice in two specific strain backgrounds. The new F2 C3H x C57Bl/6 transgenic mice will be backcrossed for seven generations with wild type inbred mice (C57Bl/6 and Balb/c) to obtain homogeneous genetic background. iii. To screen transgenic mice by molecular analyses of genomic DNA (PCR, slot blot) and provide the necessary number of mice to the investigators of the Program. The screening process will also be centralized to prevent variability and increase efficiency and reproducibility of the results from different projects. iv. To develop new applications involving the use of transgenic mice to study lung fibrosis. An alternative approach to complement the classical transgenic mouse models will be developed during the Program funding period: the generation of transgenic mice by BACs.

吸入核心的具体目的是在无病原体环境中吸入实验之前提供小鼠的壳体，并受控对石棉和卵形蛋白（OVA）的暴露。转基因小鼠核心的目的是向程序项目的成员提供转基因小鼠。新的和先前生成的传统转基因小鼠的生成，维护和筛查将集中在该核心中。此外，该核心旨在开发一种新的实验方法，涉及小鼠基因组中细菌人造染色体（BAC）稳定整合。该核心的具体目的是：i。为生成新的转基因小鼠（F2 C3H X C57BL/6）的核心服务，该小鼠表达在肺上皮细胞中的特定转基因。该计划项目中提出的新的转基因小鼠将在弗蒙特大学转基因小鼠核心（Rincon Dr. ii。集中在两个特定应变背景中获得新的和先前生成的转基因小鼠所需的后交叉过程。新型的F2 C3H X C57BL/6转基因小鼠将在七代野生型近近近交小鼠（C57BL/6和BALB/C）中进行回击，以获得均匀的遗传背景。 iii。通过基因组DNA（PCR，插槽印迹）的分子分析筛选转基因小鼠，并向该程序的研究者提供必要的小鼠。筛选过程还将集中，以防止可变性并提高不同项目结果的效率和可重复性。 iv。开发涉及使用转基因小鼠研究肺纤维化的新应用。一种补充经典转基因小鼠模型的替代方法将在程序资金期间开发：通过BAC生成转基因小鼠。