Core--Animal Model

核心--动物模型

• 批准号: 7140945
• 负责人: GARRET A FITZGERALD
• 金额: $ 15.47万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7140945
• 关键词: animal colony; aorta aneurysm; artery occlusion; atherosclerosis; biomedical facility; blood pressure; cardiovascular function; cardiovascular injury; disease /disorder model; genetically modified animals; hemodynamics; laboratory mouse; sample collection; telemetry; tissue /cell preparation

The Animal Model Core will make available to investigators a broad range of mouse models of vascular injury. These include (i) wire injury to the femoral or carotid arteries; (ii) low flow dependent model of the carotid which retains endothelial integrity and (iii) a abdominal aortic aneurysm formation in response to angiotensin II infusion in hyperlipidemic mice. Additionally, the Core is well experienced in the quantitiative and morphological analysis of atherosclerosis in diversified models of genetically prone mice. A second repertoire of models affords to assess other aspects of vascular function. Thus, continuous telemetric monitoring of the diurnal variation in blood pressure is available in conscious mice. Additionally, a model of free radical catalyzed injury to the carotid with subsequent carotid vascular occlusion is available to permit assessment of the response to a thrombogenic stimulus. The Animal Model Core is equipped for constant hemodynamic monitoring, urine collection in metabolic cages and blood sampling, cellular isolation and storage. Facilities exist for monitoring whole blood and platelet aggregation.

动物模型核心将为研究人员提供广泛的血管性小鼠模型 受伤。这些包括 (i) 股动脉或颈动脉的钢丝损伤； (ii) 低流量依赖模型 保留内皮完整性的颈动脉和（iii）腹主动脉瘤的形成 高脂血症小鼠血管紧张素 II 输注。此外，核心在定量方面拥有丰富的经验 以及多种遗传倾向小鼠模型中动脉粥样硬化的形态学分析。 第二个模型库可以评估血管功能的其他方面。因此，连续 在清醒的小鼠中可以遥测血压的昼夜变化。另外，一个 自由基催化颈动脉损伤并随后导致颈动脉血管闭塞的模型可用于 允许评估对血栓形成刺激的反应。 动物模型核心配备用于持续血流动力学监测、代谢过程中的尿液收集 笼子和血液采样、细胞分离和储存。现有监测全血和 血小板聚集。