Defining Emergence from Stationary Phase in Yeast

定义酵母从固定相出现的情况

• 批准号: 6836721
• 负责人: CHRISTOPHER P ALLEN
• 金额: $ 4.3万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836721
• 关键词: Saccharomyces cerevisiae; cell cycle; cell growth regulation; cell morphology; cytogenetics; flow cytometry; fungal genetics; gene expression; gene expression profiling; gene mutation; high throughput technology; microarray technology; postdoctoral investigator; serial analysis of gene expression; synchronous cell division; tissue /cell culture

DESCRIPTION (provided by applicant): The objective of the research proposed here is to understand quiescent cells, and the relationship between quiescent cells and mitotically cycling cells in the budding yeast Saccharomyces cerevisiae. Prior to the advent of genomics, it was not possible to study these questions at the level of the transcriptome. Using recently developed rapid-sampling technology; we can generate, for the first time, fine-resolution time course studies of gene expression in cell cultures exiting quiescence, and in exponentially dividing cell cultures. To examine quiescent cells and the relationship to cycling cells we will: 1.) Investigate the heterogeneity of stationary phase cultures with respect to cell size, DNA content, and gene expression, 2.) Conduct a rapid sampling of G1 in synchronized dividing cells, 3.) Conduct a longer time course from cells exiting stationary phase, and 4.) Develop and use mutivariate curve resolution methods as a powerful genomic analysis tool. By understanding the questions related to quiescence in yeast, we can gain significant insights into diseases that afflict humans such as the chronic infections caused by quiescent bacterial pathogens, and the carcinogenic transformation observed in cells that have lost the capacity to remain quiescent.

描述（由申请人提供）：此处提出的研究的目的是了解静止的细胞，以及在发芽​​的酵母酿酒酵母中静止细胞与有丝分裂循环细胞之间的关系。在基因组学出现之前，不可能以转录组级别研究这些问题。使用最近开发的快速采样技术；我们可以第一次生成细胞培养物中基因表达的精细分辨率时间过程，并在指数级分裂的细胞培养物中。为了检查静态细胞和与循环细胞的关系：1。）研究固定相培养的异质性，相对于细胞大小，DNA含量和基因表达，2。）在同步分裂细胞中对G1进行快速采样，3。）进行较长的时间过程，从固定相位出现和使用突变的curve curve curve curve curve santion工具有效地分析了较长的细胞。通过了解与酵母中静止有关的问题，我们可以对疾病的疾病有很大的见解，这些疾病受到了诸如静止细菌病原体引起的慢性感染以及在失去保持静态的能力的细胞中观察到的致癌转化。