Defining Emergence from Stationary Phase in Yeast

定义酵母从固定相出现的情况

• 批准号: 6933912
• 负责人: CHRISTOPHER P ALLEN
• 金额: $ 4.83万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933912
• 关键词: Saccharomyces cerevisiae; cell cycle; cell growth regulation; cell morphology; cytogenetics; flow cytometry; fungal genetics; gene expression; gene expression profiling; gene mutation; high throughput technology; microarray technology; postdoctoral investigator; serial analysis of gene expression; synchronous cell division; tissue /cell culture

DESCRIPTION (provided by applicant): The objective of the research proposed here is to understand quiescent cells, and the relationship between quiescent cells and mitotically cycling cells in the budding yeast Saccharomyces cerevisiae. Prior to the advent of genomics, it was not possible to study these questions at the level of the transcriptome. Using recently developed rapid-sampling technology; we can generate, for the first time, fine-resolution time course studies of gene expression in cell cultures exiting quiescence, and in exponentially dividing cell cultures. To examine quiescent cells and the relationship to cycling cells we will: 1.) Investigate the heterogeneity of stationary phase cultures with respect to cell size, DNA content, and gene expression, 2.) Conduct a rapid sampling of G1 in synchronized dividing cells, 3.) Conduct a longer time course from cells exiting stationary phase, and 4.) Develop and use mutivariate curve resolution methods as a powerful genomic analysis tool. By understanding the questions related to quiescence in yeast, we can gain significant insights into diseases that afflict humans such as the chronic infections caused by quiescent bacterial pathogens, and the carcinogenic transformation observed in cells that have lost the capacity to remain quiescent.

描述（由申请人提供）：本文提出的研究目的是了解出芽酵母酿酒酵母中的静止细胞以及静止细胞与有丝分裂循环细胞之间的关系。在基因组学出现之前，不可能在转录组水平上研究这些问题。采用最新开发的快速采样技术；我们首次可以对退出静止状态的细胞培养物和呈指数分裂的细胞培养物中的基因表达进行精细分辨率的时间过程研究。为了检查静止期细胞及其与循环细胞的关系，我们将：1.) 研究静止期培养物在细胞大小、DNA 含量和基因表达方面的异质性，2.) 在同步分裂细胞中进行 G1 快速采样， 3.) 从细胞退出稳定期开始进行更长的时间过程，以及 4.) 开发并使用多变量曲线解析方法作为强大的基因组分析工具。通过了解与酵母静止相关的问题，我们可以深入了解困扰人类的疾病，例如静止细菌病原体引起的慢性感染，以及在失去保持静止能力的细胞中观察到的致癌转化。