The evolution of gene expression in Drosophila

果蝇基因表达的进化

• 批准号: 6836933
• 负责人: COLIN D MEIKLEJOHN
• 金额: $ 4.3万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6836933
• 关键词: Drosophilidae; alleles; biochemical evolution; gene expression; genome; genotype; microarray technology; nucleotides; polymerase chain reaction; postdoctoral investigator; recombinant proteins

DESCRIPTION (provided by applicant): The proposed research aims to identify both the molecular mechanisms that underlie and the evolutionary processes that have produced functional divergence within gene expression networks between two closely related species. This will be initiated by experiments designed to measure gene expression across the genome for genotypes of Drosophila simulans that have had a segment of their genome replaced with the homologous segment from the closely related species Drosophila mauritiana. Identification of genes with patterns of expression in these genotype that are different from either parent species will provide insight into aspects of heterospecific interactions on gene expression. This will also provide candidate for the positional cloning of factors responsible for novel gene expression in the heterospecific genotypes. Once these factors have been identified, functional and comparative methods will be used to determine the molecular mechanisms underlying the novel expression. Finally, intraspecific genotypic and phenotypic variation within both species at loci implicated in these interactions will be obtained to identify the evolutionary forces that have led to the fixation of the incompatible alleles in these two species.

描述（由申请人提供）：拟议的研究旨在确定两个密切相关的物种之间的基因表达网络内产生功能分歧的分子机制和进化过程。这将通过旨在测量模拟果蝇基因型整个基因组的基因表达的实验来启动，这些模拟果蝇的基因组片段被密切相关的毛里求斯果蝇物种的同源片段取代。鉴定这些基因型中与任一亲本物种不同的表达模式的基因将提供对基因表达的异种特异性相互作用的深入了解。这也将为负责异种特异性基因型中新基因表达的因子的位置克隆提供候选。一旦确定了这些因素，将使用功能和比较方法来确定新表达背后的分子机制。最后，将获得两个物种内与这些相互作用有关的位点的种内基因型和表型变异，以确定导致这两个物种中不相容等位基因固定的进化力量。