Experimental evolution by precise gene replacement

通过精确基因替换进行实验进化

• 批准号: 6550829
• 负责人: ANTHONY J GREENBERG
• 金额: $ 3.83万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6550829
• 关键词: Drosophilidae; alleles; arthropod genetics; behavior test; behavioral /social science research tag; biochemical evolution; gene expression; gene rearrangement; gene targeting; genetic polymorphism; molecular genetics; natural selections; population genetics; postdoctoral investigator; reproduction; sex behavior; species difference

DESCRIPTION (provided by applicant): Studies of population genetics have been almost exclusively confined to theory and surveys of polymorphism in natural populations. Experiments in evolution have been extremely difficult to perform in a rigorous manner because established transgenic technology in most higher eukaryotes is unsuitable for such studies. Recent advances in gene knock out and transgene co-placement in Drosophila melanogaster remove most of the obstacles. We would like to put these methods together into a comprehensive framework and apply them to the study of reproductive isolation between behavioral races of D. melanogaster. Lessons learned from our experiments could then be applied to studies of complex genetic traits in other organisms. For example, many human genetic deseases, such as cancer susceptibility, are influenced by many genes.

描述（由申请人提供）：人口遗传学的研究已经 几乎仅限于自然中多态性的理论和调查 人群。进化实验非常困难 以严格的方式，因为大多数更高的转基因技术已经建立 真核生物不适合此类研究。基因的最新进展淘汰了 和果蝇中的转基因共存去除大部分 障碍。我们想将这些方法融合在一起 框架并将其应用于研究 D. Melanogaster的行为种族。从我们的实验中学到的经验教训可以 然后应用于其他生物体中复杂遗传特征的研究。为了 例如，许多人类遗传脱发，例如癌症易感性，是 受许多基因的影响。