Candida albicans-specific mucosal CD4+ T cell clones

白色念珠菌特异性粘膜 CD4 T 细胞克隆

• 批准号: 6785445
• 负责人: Welda LaJean CHAFFIN
• 金额: $ 14.8万
• 依托单位: TEXAS TECH UNIVERSITY HEALTH SCIS CENTER
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6785445
• 关键词: Candida albicans; antibody specificity; cell population study; cellular immunity; clone cells; colony stimulating factor; flow cytometry; helper T lymphocyte; host organism interaction; laboratory mouse; light microscopy; microorganism culture; microorganism growth; molecular cloning; opportunistic infections; oral bacteria; oral mucosa; pathologic process; single cell analysis; tissue /cell preparation

DESCRIPTION (provided by applicant): Candida albicans is a commensal that colonizes skin and mucosal surfaces including the oral cavity. The organism forms biofilms on mucosa and oral devices, e.g. dentures, generally in association with bacteria. The organism is also an agent of opportunistic infection of these surfaces as well as disseminated disease. Among individuals that are most susceptible to oral infection are HIV+ patients who have declining CD4+ T cells. The correlation between loss of this lymphocyte population and disease implicates CD4+ T cells in protection of the healthy individual from disease. CD4+ T cells have also been implicated in susceptibility in murine models of infection. While T-helper 1 type cell-mediated immunity (CMI) has been implicated in protection and recovery from both systemic and mucosal infection, it is also clear that mucosal and systemic immune response has elements of compartmentalization. This is emphasized by the susceptibility of the AIDS patient to oral mucosal but not systemic disease. The profile of T cells in the oral mucosa differs from that of peripheral blood. In recent years, T cells clones have been a useful tool to identify the specific antigens and their epitopes that stimulate the CMI response. However, the application of these protocols has been applied to lymphocytes of peripheral circulation and not those of the oral mucosa that are important in the response to oral pathogens. The goal of this proposal is to establish procedures for isolating CD4+ T cell clones that are derived from the T cell population of the oral mucosa in mice colonized with C. albicans. The successful development of this protocol has specific application that will provide a major tool for subsequent studies of host response and control of oral C. albicans. A successful protocol will also have more widespread application to study the mucosal response to components of the normal oral flora and agents of oral infection.

描述（由申请人提供）： 白色念珠菌是一个共同的，它在包括口腔在内的皮肤和粘膜表面定居。该生物体在粘膜和口服装置上形成生物膜，例如假牙，通常与细菌相关。生物体也是这些表面和传播疾病的机会感染的推动者。在最容易感染口腔感染的个体中，CD4+ T细胞下降的HIV+患者。这种淋巴细胞群体和疾病的丧失之间的相关性暗示了CD4+ T细胞在保护健康个体免受疾病的保护方面。 CD4+ T细胞也与鼠类感染模型中的敏感性有关。虽然T-助血器1型细胞介导的免疫（CMI）与全身性和粘膜感染的保护和恢复有关，但也很明显，粘膜和全身免疫反应具有分隔的元素。艾滋病患者对口腔粘膜的敏感性而不是全身性疾病，这强调了这一点。口服粘膜中T细胞的特征与外周血的特征不同。近年来，T细胞克隆成为识别刺激CMI反应的特定抗原及其表位的有用工具。但是，这些方案的应用已应用于外周循环的淋巴细胞，而不是口服粘膜的淋巴细胞，而不是对口腔病原体反应的重要性。该提案的目的是建立分离源自白色念珠菌定植的小鼠口服粘膜T细胞群体的CD4+ T细胞克隆的程序。该协议的成功开发具有特定的应用，该应用将为随后研究宿主反应和口服白色念珠菌的控制提供主要工具。成功的方案还将有更多的广泛应用来研究对正常口腔菌群和口腔感染药物的粘膜反应。