Candida albicans oral biofilm

白色念珠菌口腔生物膜

• 批准号: 6652571
• 负责人: Welda LaJean CHAFFIN
• 金额: $ 33.3万
• 依托单位: TEXAS TECH UNIVERSITY HEALTH SCIS CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6652571
• 关键词: Candida albicans; bacteria infection mechanism; biofilm; clinical research; computer data analysis; fungal genetics; gene environment interaction; gene expression; human tissue; microarray technology; mycosis; nucleic acid probes; opportunistic infections; oral bacteria; polymerase chain reaction; regulatory gene; scanning electron microscopy

DESCRIPTION (provided by applicant): Candida albicans is a commensal that colonizes skin and mucosal surfaces including the oral cavity. The organism is also an agent of opportunistic disease of these surfaces as well as internal disseminated disease. Oral candidiasis is associated with derangements of the oral flora related with the acquisition of microbes by neonates and anti-bacterial therapy, oral prostheses, and host factors such as diabetes mellitus and HIV infection. Oral manifestations include pseudomembraneous candidiasis (thrush) and denture stomatitis. Oropharyngeal infection is virtually an inescapable consequence of AIDS (96 percent patients) and frequently reoccurs. Denture stomatitis may affect 50 percent of complete denture wearers. The organism forms biofilms on mucosa, teeth and oral devices such as dentures, generally in association with oral bacteria. Compared to planktonic cells, organisms in biofilms have characteristics such as reduced susceptibility to antifungal drugs and the presence of an extracellular matrix. This study will test the hypothesis that unique characteristics associated with C. albicans biofilms are the result of altered gene expression in general cellular metabolism as well as bioflim specific gene expression. A model of saliva-coated denture acrylic established in this laboratory will be used. About 230 alterations in general cellular metabolism have been identified in biofilm compared to planktonic cells by exploiting the high homology between Saccharomyces cerevisiae and C. albicans and the commercial availability of gene arrays for S. cerevisiae. In Aim 1 this approach will be applied to examine expression temporally during biofilm formation and to other conditions of biofilm development using C. albicans DNA chips. In Aim 2, expression in in vitro biodiverse models will also be examined to identify genes inherently associated with biofilms as differentiated from those influenced by the biofilm environment. Expression of selected genes from the inherent biofilm expression class will be determined in vivo in organisms recovered from human saliva. Aim 3 will examine the role of biofilm-regulated genes such as TUP1and EFG1 using genetically modified strains.

描述（由申请人提供）：白色念珠菌是一个共同点 在包括口腔在内的皮肤和粘膜表面定居。有机体是 也是这些表面的机会性疾病的推动者以及内部的 传播疾病。口服念珠菌病与 口腔菌群与新生儿的微生物相关，并 抗菌疗法，口腔假体和宿主因素（例如糖尿病） Mellitus和HIV感染。口服表现包括假膜 念珠菌病（鹅口疮）和义齿的气孔炎。口咽感染是 几乎是艾滋病（96％的患者）和 经常再次出现。义齿的气孔炎可能影响50％的完整 义齿佩戴者。生物体在粘膜，牙齿和口服装置上形成生物膜 例如假牙，通常与口腔细菌相关。相比 浮游细胞，生物膜中的生物具有降低的特征 对抗真菌药物的敏感性和细胞外基质的存在。 这项研究将检验以下假设 白色念珠菌生物膜是基因表达改变的结果 细胞代谢以及生物叶状特异性基因表达。一个模型 将使用在本实验室建立的唾液涂层丙烯酸。 在一般细胞代谢中大约有230种改变已在 与浮游细胞相比，生物膜通过利用高同源性 酿酒酵母和白色念珠菌以及商业可用性 酿酒酵母的基因阵列。在AIM 1中，此方法将应用于 在生物膜形成期间和其他条件期间暂时检查表达 使用白色念珠菌DNA芯片开发生物膜的。在AIM 2中，表达在 还将检查体外生物多样性模型以固有地识别基因 与生物膜影响的生物膜有关 环境。从固有生物膜表达中的选定基因表达 在从人类唾液中回收的生物体中，将确定类体内确定的。目的 3将检查使用生物膜调节的基因（例如TUP1和EFG1）的作用 转基因菌株。