PHOTOPOLYMERIZATION FOR NONINVASIVE CARTILAGE TISSUE

非侵入性软骨组织的光聚合

• 批准号: 6516531
• 负责人: ROBERT Samuel LANGER
• 金额: $ 32.7万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6516531
• 关键词: athymic mouse; biomaterial compatibility; biomaterial development /preparation; cartilage disorder; cartilage transplantation; dogs; gel; growth factor; implant; laboratory mouse; medical implant science; medical rehabilitation related tag; membrane permeability; oral facial restoration; oral facial restoration material; photochemistry; polymerization; skin absorption; swine; tissue engineering; transdermal drug delivery

DESCRIPTION (Adapted from the applicant's abstract) The objective of this grant proposal is to develop a noninvasive method to implant polymers using photopolymerization. This allows solid hydrogels to be implanted without surgical intervention. Enough light is able to penetrate tissue, including skin, to trigger the photopolymerization of an injected liquid, polymer solution to a solid hydrogel. Further study of transdermal photopolymerization using alternative photoinitiating systems and wavelengths of light is proposed in order to increase depths at which implants can be photopolymerized. Photopolymerization will be applied to the tissue engineering of cartilage. Cartilage lacks the ability to regenerate and its loss by trauma, congenital abnormalities or tumors gives few options to the physician for replacement. Preliminary data has shown that chondrocytes survive encapsulation, injection and photopolymerization to form neocartilage. Important biological factors, including growth factors and adhesion peptides, will be incorporated into photopolymerizing hydrogels to determine if they can accelerate cartilage development and produce cartilage with biochemical and biomechanical properties similar to native cartilage. The tissue engineering of cartilage will be examined both in vitro and in vivo, in particular in an immune competent animal. The integration of photopolymerized tissue engineered cell/polymer implants with surrounding native cartilage is critical to the clinical application of tissue engineered cartilage and will be addressed. In conclusion, this proposal addresses methods for noninvasive polymer implantation, which applied to cartilage tissue engineering, would provide physicians with a significant alternative to cartilage replacement for craniofacial reconstruction and orthopedic surgery. The specific goals of this proposal include: (1) development of a highly efficient, biocompatible transdermal or transtissue photopolymerization system for noninvasive polymer implantation; (2) development of an injectable tissue engineered cartilage using transdermal photopolymerization; (3) increase cellular biocompatibility of the polymer hydrogel through the incorporation of bioactive peptides and proteins; and (4) integration of tissue engineered and native cartilage for the correction of cartilage defects.

描述 （改编自申请人的摘要）本拨款提案的目标 是开发一种非侵入性方法来植入聚合物 光聚合。 这使得固体水凝胶无需植入即可植入 手术干预。 足够的光能够穿透组织，包括 皮肤，触发注射液体聚合物的光聚合 溶液成固体水凝胶。 透皮吸收的进一步研究 使用替代光引发系统的光聚合和 提出了光的波长，以增加深度 植入物可以光聚合。 光聚合将应用于 软骨组织工程。 软骨缺乏能力 再生及其因创伤、先天异常或肿瘤而丧失 医生更换的选择很少。 初步数据显示 软骨细胞在封装、注射和光聚合中存活 形成新软骨。 重要的生物因素，包括生长 因子和粘附肽，将被纳入光聚合 水凝胶以确定它们是否可以加速软骨发育 产生具有类似于的生化和生物力学特性的软骨 原生软骨。 软骨的组织工程将被检查 体外和体内，特别是在免疫能力强的动物中。 这 光聚合组织工程细胞/聚合物植入物与 周围的天然软骨对于临床应用至关重要 组织工程软骨将得到解决。 综上所述，这 该提案涉及非侵入性聚合物植入的方法，该方法 应用于软骨组织工程，将为医生提供 颅面软骨置换的重要替代方案 重建和骨科手术。 本提案的具体目标包括：（1）发展高度 高效、生物相容性透皮或跨组织光聚合 非侵入性聚合物植入系统； (2) 开发 使用透皮注射的组织工程软骨 光聚合； (3)增加聚合物的细胞生物相容性 通过掺入生物活性肽和蛋白质形成水凝胶；和 (4) 组织工程软骨与天然软骨的整合进行矫正 软骨缺损。