In situ Crosslinkable Hydrogels for Tissue Repair

用于组织修复的原位可交联水凝胶

• 批准号: 6690407
• 负责人: NANCY JANE SHELBY
• 金额: $ 10.7万
• 依托单位: WYOMING RESEARCH INNOVATIONS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6690407
• 关键词: athymic mouse; biomaterial compatibility; biomaterial development /preparation; cell proliferation; chondroitin sulfates; collagen; crosslink; elastin; extracellular matrix; fibronectins; fluorescence microscopy; gel; gelatin; hyaluronate; mucopolysaccharides; regeneration; thiols; tissue engineering; wound healing

DESCRIPTION (provided by applicant): Wyoming Research Innovations, Inc. will develop in situ cross-linkable hydrogels, called synthetic extracellular matrix (sECM), for soft tissue repair. The ability to fill a traumatic, surgical, or chronic defect with a self-molding cell-interactive sECM prepared with a fast-curing mixture of biocompatible macromonomers would have significant clinical utility. These materials will be prepared from chemically modified hyaluronic acid and chondroitin sulfate, which are two major glycosaminoglycan (GAG) components of the ECM. Biomaterials from cross-linked GAG derivatives produce versatile new bioinspired materials. The sECM concept incorporates ECM proteins as well as GAGs, and we propose to demonstrate that crosslinking can occur in the presence of seeded cells and in a tissue defect in vivo. Two crosslinking chemistries will be investigated and the resulting materials will be evaluated for compatibility with cell growth. In situ cross-linked materials will be evaluated for in vivo efficacy in an animal model. The proposed materials address an unmet need in surgical care and wound repair. Many surgical defects, traumatic wounds and deep chronic wounds fail to heal without augmentation using grafted natural or synthetic materials. A biocompatible hydrogel that mimics ECM and is conducive to cell seeding and growth would have many important clinical applications.

描述（由申请人提供）： 怀俄明州研究创新公司将开发原位可交联水凝胶，称为合成细胞外基质（sECM），用于软组织修复。使用由生物相容性大分子单体的快速固化混合物制备的自成型细胞交互性 sECM 来填充创伤性、手术性或慢性缺陷的能力将具有重要的临床实用性。这些材料将由化学修饰的透明质酸和硫酸软骨素制成，它们是 ECM 的两种主要糖胺聚糖 (GAG) 成分。来自交联 GAG 衍生物的生物材料可生产多功能的新型仿生材料。 sECM 概念结合了 ECM 蛋白和 GAG，我们建议证明交联可以在存在种子细胞和体内组织缺陷的情况下发生。将研究两种交联化学物质，并评估所得材料与细胞生长的相容性。原位交联材料将在动物模型中评估体内功效。 拟议的材料解决了外科护理和伤口修复方面未满足的需求。如果不使用移植的天然或合成材料进行增强，许多手术缺陷、创伤性伤口和深层慢性伤口就无法愈合。模拟 ECM 并有利于细胞播种和生长的生物相容性水凝胶将具有许多重要的临床应用。