Automated Monitoring of Chemotactic Cell Movement

趋化细胞运动的自动监测

• 批准号: 6406033
• 负责人: MICHAEL A LYNES
• 金额: $ 24.4万
• 依托单位: APPLIED BIOPHYSICS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2002-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6406033
• 关键词: biomedical automation; biomedical equipment development; cell line; cell migration; chemoattractants; chemotaxis; electrical impedance; electrodes; high throughput technology; laboratory mouse; monitoring device

DESCRIPTION ( provided by applicant): The ECIS/taxis technology can detect cells that move to a small electrode through an agarose matrix by the resistance that they impart to an electrical circuit as they cover parts of the small electrode. This system is amenable to rapid throughput analysis of cell behavior in the presence of chemokines and other agents that motivate cell movement. This technology has important advantages as an alternative to traditional measures of cell movement. First, it enables the evaluation of cell responses to stimuli without the preestablishment of a specific endpoint. Second, the ECIS/taxis environment more closely reflects the natural biological environment. Third, the data can be quantitatively assessed according to changes in the resistance of the circuit. Our result in the Phase I of this project clearly demonstrate that this technology can be used to measure the response of cells to chemotactic factors and effects of drugs on the chemotactic response. In Phase II of this project, we will develop the protocols that can be used to evaluate the chemotactic and chemokinetic responses of mammalian leukocytes, and the methods of evaluation of prototype antagonists of chemokinesis. PROPOSED COMMERCIAL APPLICATION: The ECIS/taxis technology will be immediately useful in the search for anti-chemotactic agent (anti-inflammatory agents), chemotactic agents (new chemokines), agonists of known chemokines (pro-inflammatory agents), and stimulators of motility (pro-inflammatory agents).

描述（由申请人提供）：ECIS/出租车技术可以检测 细胞通过琼脂糖基质移动到小电极 当它们覆盖电路的一部分时，它们会给电路带来电阻 小电极。该系统适合细胞的快速通量分析 在趋化因子和其他刺激细胞的物质存在下的行为 移动。该技术作为替代技术具有重要优势 细胞运动的传统测量方法。首先，它能够评估细胞 在没有预先设定特定终点的情况下对刺激的反应。 其次，ECIS/出租车环境更贴近地反映自然生物 环境。第三，可以根据数据进行定量评估 电路电阻的变化。我们在第一阶段的结果 项目清楚地表明该技术可用于测量 细胞对趋化因子的反应以及药物对趋化因子的影响 趋化反应。在该项目的第二阶段，我们将开发 可用于评估趋化和趋化动力学的方案 哺乳动物白细胞反应及原型评价方法 趋化作用的拮抗剂。 拟议的商业应用： ECIS/出租车技术将立即用于寻找抗趋化药物 剂（抗炎剂）、趋化剂（新趋化因子）、激动剂 已知的趋化因子（促炎剂）和运动刺激剂（促炎剂） 代理）。