SECOND MOLECULAR FORM OF GONADOTROPIN RELEASING HORMONE IN PRIMATES

灵长类动物促性腺激素释放激素的第二种分子形式

• 批准号: 6592282
• 负责人: HENRYK F URBANSKI
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592282
• 关键词: Mammalia; endocrine gland /system; growth /development; nervous system; reproductive system

In mammals, reproduction is thought to be controlled by a single neuropeptide, gonadotropin-releasing hormone (GnRH-I), which regulates the synthesis and secretion of gonadotropins from the pituitary gland. However, another form of this decapeptide (GnRH-II), of unknown function, also exists in the brain of many vertebrate species, including humans; it is encoded by a different gene and its amino acid sequence is 70% identical to that of GnRH-I. We have now cloned GnRH-II cDNA from the rhesus macaque (Macaca mulatta), and have shown by in situ hybridization that GnRH-II mRNA is expressed in the primate midbrain, hippocampus and discrete nuclei of the hypothalamus, including the supraoptic, paraventricular, suprachiasmatic and arcuate. Because the regional distribution pattern of cells containing GnRH-II mRNA is largely dissimilar to that of cells containing GnRH-I mRNA, it is likely that these two cell populations receive distinct neuroendocrine inputs and thus regu late GnR H synthesis and release differently. Elucidation of how GnRH neurons are integrated with major neurotransmitters of the central nervous system is fundamental to the understanding of hypothalamic causes of human infertility and reproductive disorders such as precocious and delayed puberty. Therefore, these data should help in the design of therapies and also provide new approaches for contraception. FUNDING Medical Research Foundation of Oregon PUBLICATIONS Urbanski HF, Garyfallou VT, Rodrigues SM, Kohama SG. Subpopulation specificity in the capacity of rhesus macaque GnRH neurons to express GluR1. Soc Neurosci Abstr 24:270, 1998 (abstract 110.7). White RB, Urbanski HF, Fernald RD. A second gene for gonadotropin-releasing hormone is expressed in the rhesus macaque. Soc Neurosci Abstr 24:1609, 1998 (abstract 632.18).

在哺乳动物中，繁殖被认为是由单一的控制 神经肽，促性腺激素释放激素（GnRH-I），调节 垂体合成和分泌促性腺激素。 然而，这种十肽的另一种形式（GnRH-II）的未知数 功能，也存在于许多脊椎动物的大脑中， 包括人类；它由不同的基因及其氨基酸编码 序列与 GnRH-I 的序列有 70% 相同。 我们现在已经克隆了 来自恒河猴 (Macaca mulatta) 的 GnRH-II cDNA，并已显示 通过原位杂交发现 GnRH-II mRNA 在灵长类动物中表达 中脑、海马体和下丘脑的离散核团， 包括视上、室旁、视交叉上和 弓形的。 由于细胞的区域分布格局 含有 GnRH-II mRNA 的细胞与细胞有很大不同 含有 GnRH-I mRNA，这两个细胞群很可能 接受不同的神经内分泌输入，从而调节 GnR H 合成和释放不同。 阐明 GnRH 神经元如何 与中枢神经的主要神经递质整合 系统是理解下丘脑原因的基础 人类不孕症和生殖障碍，例如早熟和 青春期延迟。 因此，这些数据应该有助于设计 疗法，还提供了新的避孕方法。 资金 俄勒冈医学研究基金会出版物 Urbanski HF, 加里法卢 VT、罗德里格斯 SM、科哈马 SG。 亚群特异性 恒河猴 GnRH 神经元表达 GluR1 的能力。 社会组织 Neurosci Abstr 24:270，1998（摘要 110.7）。 白色 RB、Urbanski HF、 费尔纳德路。 促性腺激素释放激素的第二个基因是 在恒河猴中表达。 社会神经科学摘要 24:1609, 1998 （摘要 632.18）。