NEURAL CONTRIBUTION TO POLYCYSTIC OVARIAN SYNDROME

神经系统对多囊卵巢综合征的影响

• 批准号: 6592278
• 负责人: Sergio R Ojeda
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592278
• 关键词: Mammalia; animal tissue; clinical research; endocrine gland /system; gene therapy; growth /development; nervous system; reproductive system; women's health

The goal of this project was to obtain preliminary evidence for the development of a nonhuman primate model of polycystic ovarian syndrome (PCOS). The 4 monkeys used for the experiment had cycled consistently over the previous 12 months (12 or 13 menses recorded, at regular intervals). They were provided with an intraovarian graft of genetically modified baby hamster kidney cells, encapsulated in a polymer of poly [acrylonitrile vinyl chloride, P(AN-VC)]. The surgical implants were performed as close to the first day of the new menses cycle as possible. Two of the monkeys received devices which contained NGF-secreting cells and two of the monkeys received control devices containing unmodified cells. The implants were 0.7 mm outside diameter and 7 mm long. Each monkey received two implants in each ovary. Following laparotomy to expose the ovaries, the implants were inserted into the ovary using a large bore needle and plunger (a suture in the ovarian capsule prevented migration of the device). Following implantation, all four monkeys continued to cycle and did so throughout the six month study; thus, the implants themselves did not affect normal ovarian function. The collected serum samples were assayed for ovarian steroids and luteinizing hormone. Of the steroids analyzed (estradiol, progesterone, testosterone and androstenedione), only androstenedione was elevated following the grafting of NGF producing cells; the other steroids remained constant. Luteinizing hormone levels were also elevated in the monkeys that received the NGF producing grafts. Androstenedione and LH are the two hormones most consistently linked with PCOS in humans. The ovarian morphology following grafting is still being assessed; however, initial observations suggest a possible increase in the number of follicular structures, again reminiscent of PCOS. Although further analysis of the data is necessary, preliminary evaluation suggests that an elevation of intraovarian N GF levels may be one of the components required to generate a nonhuman primate model of PCOS. FUNDING Center-supported project PUBLICATIONS None

该项目的目标是获得初步证据 非人灵长类多囊卵巢模型的建立 综合征（多囊卵巢综合症）。 用于实验的4只猴子骑自行车 过去 12 个月内持续出现月经（记录了 12 或 13 次月经， 定期间隔）。 他们接受了卵巢内移植 转基因小仓鼠肾细胞，封装在 聚[丙烯腈氯乙烯，P(AN-VC)]的聚合物。 这 手术植入是在接近新生命的第一天进行的 月经周期尽可能。 其中两只猴子收到的设备 含有 NGF 分泌细胞，其中两只猴子接受了对照 含有未修改细胞的设备。 种植体外侧 0.7 毫米 直径7毫米，长7毫米。 每只猴子的每个部位都植入了两个植入物 子房。 剖腹手术暴露卵巢后，将植入物 使用大孔针和柱塞（a 卵巢囊中的缝合阻止了装置的迁移）。 植入后，所有四只猴子都继续骑自行车并这样做 整个六个月的研究；因此，植入物本身并没有 影响卵巢正常功能。 采集的血清样本为 检测卵巢类固醇和黄体生成素。 类固醇的 分析（雌二醇、黄体酮、睾酮和雄烯二酮）， NGF 移植后仅雄烯二酮升高 生产细胞；其他类固醇保持不变。 黄体化 接受 NGF 的猴子的激素水平也有所升高 产生移植物。 雄烯二酮和 LH 是最重要的两种激素 一直与人类多囊卵巢综合症有关。 卵巢形态 嫁接后的情况仍在评估中；然而，最初 观察结果表明，卵泡数量可能增加 结构，再次让人想起多囊卵巢综合症。 虽然进一步分析 该数据是必要的，初步评估表明 卵巢内 N GF 水平升高可能是其中之一 生成 PCOS 的非人类灵长类动物模型所需。 资金 中心资助项目 出版物 无