CRYSTAL STRUCTURE OF FRAGMENTS OF HEAT SHOCK TRANSCRIPTION FACTOR

热休克转录因子片段的晶体结构

• 批准号: 6658550
• 负责人: HILLARY C NELSON
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658550
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

We have determined the structure of the DNA binding domain of the heat shock transcription factor (HSF) from Kluyveromyces lactis. Given the 1.05 E resolution of the structure, there are many detailed questions that can be addressed. First, we are interested in an unusual helix that contains a proline (which causes a kink) and an extra amino acid (which causes a bulge). Crystals have been obtained that have an alanine instead of the proline and the structure determination is underway. Second, we are interested in how the protein binds DNA. Crystals have been obtained of a complex between the DNA binding domain and its cognate binding site. The data collected on beamline 7-1 was used along with MAD data collected on 1-5 to determine the structure of the complex.

我们已经确定了 DNA 结合域的结构 来自乳酸克鲁维酵母的热休克转录因子（HSF）。 鉴于该结构的分辨率为1.05 E，有许多详细信息 可以解决的问题。 首先，我们感兴趣的是 不寻常的螺旋，含有脯氨酸（导致扭结）和 额外的氨基酸（导致凸起）。 已获得结晶 具有丙氨酸而不是脯氨酸和结构 决定正在进行中。 其次，我们感兴趣的是如何 蛋白质与DNA结合。 已获得以下复合物的晶体 DNA 结合域及其同源结合位点。 数据 在光束线 7-1 上收集的数据与在 1-5确定复合物的结构。