SELENIUM & BROMINE DOUBLE EDGE MAD EXPERIMENT FOR AN HSF DNA COMPLEX

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• 批准号: 6586596
• 负责人: HILLARY C NELSON
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586596
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

We have used multiple anomalous disperson (MAD) to determine the structure of a complex between the DNA binding domain of the heat shock transcription factor (HSF) along with a 12-mer of DNA containing its cognate binding site. Data was collected on crystals containing selenomethionine-substituted protein and bromouracil-substituted DNA. The present model contains complete DNA, most of the protein, and 100 water molecules. The protein monomers are missing residues from the N- and C-terminii, as well as from the flexible loop. The current R value is 21.0% (free R is 29.3%) for all data between 6.0 and 2.5 E. There are no Ramachandran outliers, the r.m.s deviations are 0.009 E for bond lengths and 1.5540 for bond angles. The structure shows that the DNA is contacted solely along with phosphate backbone, with the majority of contacts coming from the putative recognition helix of the DNA binding domain. We are in the process of interpreting the biological implications of the structure.

我们已经使用了多个异常的驱动器（MAD）来确定 热的DNA结合结构域之间的复合物的结构 冲击转录因子（HSF）以及12-Mer含有DNA 它的同源结合位点。 数据是在包含晶体上收集的 硒蛋白蛋白取代的蛋白质和溴酸溶质取代的DNA。 本模型包含完整的DNA，大多数蛋白质和100 水分子。 蛋白质单体缺少来自 n-和c-terminii，以及柔性循环。 当前r 对于6.0和2.5 E.的所有数据，值为21.0％（免费R为29.3％）。 没有Ramachandran异常值，R.M.S的偏差为0.009 E 键长的键长为1.5540。 结构表明 DNA仅与磷酸骨架一起接触， 大多数联系来自推定的识别螺旋 DNA结合域。 我们正在解释 结构的生物学意义。