CRYSTALLOGRAPHY OF RIBOSOMES

核糖体晶体学

• 批准号: 6658515
• 负责人: ADA E YONATH
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658515
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

X-ray diffraction data are being collected best to 2.9 E from crystals of ribosomes, their complexes, mimicking defined functional states (e.g., 70S ribosomes with a short mRNA and two charged tRNA molecules), their native and their mutated, depleted and modified subunits, at cryo-temperature, using intense synchrotron radiation sources. Quantitative multi-metal cluster binding led to an SIR map at low resolution. Data collected from crystals soaked in solutions of multi-metal salts, led to an interpretable difference Patterson map at medium resolution. Parallel low resolution phasing by direct methods, rotation searches and solvent contrast variation, led to the construction of an envelope, showing striking similarity to that observed by electron microscopy.

X射线衍射数据最能收集到2.9 E 核糖体的晶体，它们的复合物，模仿定义的功能 状态（例如，70S核糖体，带有短mRNA和两个带电的tRNA 分子），它们的本地和突变，耗尽和修饰 在冷温下，亚基使用强烈的同步加速器辐射 来源。 定量多金属簇结合导致SIR地图 低分辨率。 从溶液中浸泡的晶体收集的数据 多金属盐，导致了可解释的差异帕特森地图 在中等分辨率下。 平行低分辨率通过直接逐步分阶 方法，旋转搜索和溶剂对比变化，导致了 信封的构造，显示出与之相似的相似之处 通过电子显微镜观察。