CRYSTALLOGRAPHIC STUDIES ON RIBOSOMES

核糖体的晶体学研究

• 批准号: 6220531
• 负责人: ADA E YONATH
• 金额: $ 4.84万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-15 至 2000-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220531
• 关键词: bioimaging /biomedical imaging; biological products; biomedical resource; proteins; radiography; structural biology

We have determined the structure of intact human rhinovirus 14 complexed with neutralizing Fab fragments to ast least 4.5
resolution. This application is for more CHESS time to extend the resolution of these studies by either refining freezing conditions or by complexing the virus with VH-VL fragments. This represents the first crystal structure of an intact, antibody-neutralized virus. This structure will finally address the mechanism first proposed by Mandel in 1976 where antibodies act solely on virions by inducing conformational changes in the virion. Bovine glutamate dehydrogenase is a highly allosteric homo-hexameric enzyme that catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate. The enzyme is affected by numerous allosteric effectors and has unusual kinetic properties. We have crystallized the enzyme as an abortive complex along with the inhibitor GTP and the crystals diffract to ~2.7
resolution. These fragile crystals require synchrotron time to collect a native data set.

我们已经确定了完整的人鼻病毒的结构14 与中和的Fab碎片复合至至少4.5 解决。 此应用程序是为了扩展更多的国际象棋时间 通过完善冰冻条件或 通过将病毒与VH-VL片段复合。 这代表 完整的抗体中和病毒的第一晶体结构。 这种结构最终将解决最初提出的机制 曼德尔（Mandel）在1976年，抗体仅通过诱导病毒体作用 病毒体的构象变化。 牛谷氨酸脱氢酶 是一种高度变构的同型己酸酶，可催化 谷氨酸甲酸二酮酸盐的可逆氧化脱氨基。 这 酶受许多变构效应子的影响，并且具有不寻常的 动力学特性。 我们已经将酶结晶为流产 复合物与抑制剂GTP和晶体衍射到 〜2.7分辨率。 这些脆弱的晶体需要同步时间到 收集本地数据集。