HIGH RESOLUTION MN KB FLUORESCENCE

高分辨率 MN KB 荧光

• 批准号: 6586706
• 负责人: Stephen P. Cramer
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586706
• 关键词: biomaterials; biomedical resource; spectrometry; structural biology; technology /technique

With the high resolution fluorescence spectrometers (for Mn and Fe) developed in this lab, we have obtained the basic knowledge of the chemical environments of the Mn and Fe model systems as well as refined the spectrometer. The information revealed from the kb fluorescence dependence on the spin states, oxidation states and ligand field strength can be used for studying more complicated protein systems. We would like to apply this new technique to the Photo System II in order to determine the electronic structures of the intermediate states and to nitrogenase to study the active sites and the interactions between them. We would also like to have a chance to develop a spectrometer for Mo, which will help to solve the puzzle of how the Mo functions during the catalytic process in nitrogenase.

配备高分辨率荧光光谱仪（针对 Mn 和 Fe）在本实验室开发，我们已经获得了基本知识 Mn 和 Fe 模型系统的化学环境以及 改进了光谱仪。 据kb透露的信息 荧光依赖于自旋态、氧化态和 配体场强可用于研究更复杂的 蛋白质系统。 我们希望将这项新技术应用到 Photo System II 用于确定电子结构 中间态和固氮酶来研究活性位点和 他们之间的相互作用。 我们也希望有机会 开发Mo的光谱仪，这将有助于解决Mo的难题 Mo 在固氮酶催化过程中如何发挥作用。