HUMAN REPLICATION PROTEIN COMPLEXED W/ SINGLE STRANDED DNA

与单链 DNA 复合的人类复制蛋白

• 批准号: 6658585
• 负责人: ALED M EDWARDS
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658585
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

Replication protein A (RPA) is a key component of the DNA replication and repair machineries. RPA is a single stranded DNA binding protein that interacts with DNA replication origin binding proteins, DNA polymerases, transcription activator proteins and DNA repair factors, including those that are mutated in several forms of human cancer. RPA is a heterotrimer; the DNA binding domain has been localized to the largest subunit. We have used limited proteolysis and deletion analysis to identify the minimal single strand DNA binding domain. We have crystallized this domain together with ssDNA. We are pursuing heavy atom searches on our unit and have also prepared selenomethionine-labeled protein for co-crystallization.

复制蛋白A（RPA）是DNA的关键组成部分 复制和维修机械。 RPA是单个链DNA 与DNA复制起源结合相互作用的结合蛋白 蛋白质，DNA聚合酶，转录激活蛋白和DNA 维修因素，包括以几种形式突变的因素 人类癌。 RPA是异三聚体； DNA结合域已 定位于最大的亚基。 我们使用了有限的蛋白水解 和删除分析以识别最小的单链DNA 结合域。 我们已经与ssDNA结晶了这个域。 我们正在对我们的单位进行大量的原子搜索，还准备了 用于共结晶的硒甲米氨酸标记的蛋白质。