Core B - Biomarkers Core

核心 B - 生物标志物核心

基本信息

批准号：
10005911
负责人：
Garry R Buettner
金额：
$ 27.51万
依托单位：
UNIVERSITY OF IOWA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-19 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005911
关键词：
Address Adjuvant Basic Science Biochemical Biochemical Markers Biological Biological Assay Biological Markers Biological Sciences Biology Buffers Cancer Patient Cell Culture Techniques Cell Respiration Cells Citric Acid Cycle Clinical Trials Communities Complex Comprehensive Cancer Center Core Facility Couples Data Development Electron Transport Energy Metabolism Ensure Excision Exposure to Ferritin Free Radicals Genus Hippocampus Glucose Goals Hydrogen Peroxide Ionizing radiation Ions Iowa Iron Lead Liquid substance Measurement Medicine Metabolism Metals Methodology Methods Mitochondria Molecular Biology Monitor Normal tissue morphology Oxidation-Reduction Pathway interactions Patients Peroxides Pharmacologic Ascorbate Pharmacology Study Plasma Pre-Clinical Model Procedures Production Proteins Reagent Reproducibility Research Research Personnel Sampling Scientific Advances and Accomplishments Services Source Standardization System Technology Therapeutic Time Tissues Transferrin Universities antioxidant enzyme ascorbate cancer therapy chemoradiation college cost effective data dissemination dosimetry experience experimental study high standard improved outcome innovation metal transporting protein 1 oxidative damage pre-clinical preclinical study reagent standard small molecule synergism therapy design tissue injury tool

项目摘要

Project Summary/Abstract - Core B: The goal of the Biomarkers Core (Core B) is to support Projects 1-3 and Core C in this P01 application by providing bioanalytical support for a broad range of analytical and biochemical assays that address the fundamental redox biology of the production of O2-/H2O2, hydroperoxide metabolism, and processing of redox active metal ions relevant to the study of pharmacological ascorbate (P-AscH-) in preclinical models and clinical trials. Core B will provide validated reagents, e.g. stock solutions of key reagents, standards, Seahorse and EPR analyses, enzymological analyses, ionizing radiation services, and molecular biology reagents, to Projects 1-3. Examples range from our unique, efficient, and cost-effective assays for determining plasma ascorbate concentrations in patient-derived samples (Project 1 and Project 2) as well as assays for ascorbate in the wide variety of samples generated in the proposed preclinical studies (Projects 1-3). Core B has a large repertoire of assays that will examine the basic redox biology of P-AscH-. The leaders of the core have also developed new unique assays and methods that are focused on addressing questions central to the proposed clinical trials in this P01. The leaders have more than 30 years of experience in this field as well as in directing University of Iowa core facilities in the Carver College of Medicine and the Holden Comprehensive Cancer Center. Thus, they are well qualified to lead this core.

项目摘要/摘要 - 核心 B：生物标记核心（核心 B）的目标是通过以下方式支持此 P01 应用程序中的项目 1-3 和核心 C：为广泛的分析和生化测定提供生物分析支持，以解决 O2/H2O2 产生、氢过氧化物代谢和氧化还原加工的基本氧化还原生物学与临床前模型中药理学抗坏血酸（P-AscH-）研究相关的活性金属离子和核心 B 将验证所提供的试剂，例如关键试剂的储备溶液、标准品、海马和 EPR 分析、酶学分析、电离辐射服务和分子生物学试剂，到项目 1-3 的示例范围包括我们独特、高效且具有成本效益的测定方法。患者来源样本中的血浆抗坏血酸浓度（项目 1 和项目 2）以及检测在拟议的临床前研究（项目 1-3）中生成的各种样品中都含有抗坏血酸。拥有大量检测方法，可检查 P-AscH- 核心领导者的基本氧化还原生物学。还开发了新的独特的分析方法和方法，重点解决与该 P01 的临床试验提议的领导者在该领域拥有 30 多年的经验。指导爱荷华大学卡弗医学院和霍顿综合医院的核心设施因此，他们完全有资格领导这个核心。