REGULATION OF FUNCTIONAL RECOVERY AFTER SPINAL INJURY

脊柱损伤后功能恢复的调节

• 批准号: 6363962
• 负责人: AVIS H COHEN
• 金额: $ 21.34万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-28 至 2004-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6363962
• 关键词: Agnatha; brain stem; dopamine; dorsal root; fiber cell; gamma aminobutyrate; gene expression; glycine; immunocytochemistry; nervous system regeneration; neural degeneration; neuroanatomy; neuronal guidance; neurons; neurophysiology; neuroregulation; neurotransmitters; radiotracer; serotonin; spinal cord injury; stainings

DESCRIPTION: (Verbatim from the Applicant's Abstract) The larval lamprey exhibits axonal regeneration and functional recovery following spinal cord lesions. However, the PI has found that by altering the temperature at which animals are maintained, it is possible to produce animals that exhibit either functional or dysfunctional swimming behavior. Thus the lamprey offers the opportunity to study the nature and consequences of dysfunctional regeneration. She has further found anatomical differences between the regenerated systems under the two conditions, specifically in terms of the distribution of serotoninergic axons. Following transections, there is a dramatic increase in serotonin fibers in areas rostral to the lesion, and a dramatic loss in caudal segments. However under conditions that favor recovery, serotonin fibers are more abundant in the caudal segments. She hypothesizes that serotonin fibers are responsible for at least one form of the behavioral differences seen under the different temperature conditions. The PI now proposes to identify the sources of the increased serotonin innervation in rostral segments, and the fate of the lost serotonin in caudal segments. One possibility that will be explored is that the increases in caudal segments is due to collareral sprouting of descending serotonin systems. She will test this hypothesis by evaluating whether the serotonin fibers originate from the brainstem or from dorsal roots. The fate of dopamine fibers will also be evaluated. Other studies will evaluate the functional capabilities of the animals using a sophisticated stochastic phase model to evaluate physiological responses. This technique, which has been developed by the PI, provides a quantitative measure of the degree of coordination between segments. In the same animals, the regenerated fibers will be traced anatomically and using specific markers for GABA and glycine.

描述：（逐字摘自申请人摘要）七鳃鳗幼虫 脊髓后表现出轴突再生和功能恢复 病变。然而，PI 发现通过改变温度 饲养动物，有可能产生具有以下任一特征的动物： 功能性或功能性游泳行为。因此七鳃鳗提供了 有机会研究功能失调的再生的性质和后果。 她进一步发现了再生系统之间的解剖学差异 在这两种情况下，特别是在分布方面 血清素能轴突。横断面之后，数量急剧增加 病变头侧区域的血清素纤维减少，尾侧区域急剧减少 段。然而，在有利于恢复的条件下，血清素纤维 尾节更丰富。她推测血清素纤维 造成至少一种形式的行为差异 不同的温度条件。 PI 现在建议确定血清素增加的来源 头侧节段的神经支配，以及尾侧丢失的血清素的命运 段。将探讨的一种可能性是尾部的增加 节段是由于下降的血清素系统的领口萌芽所致。她 将通过评估血清素纤维是否起源来检验这一假设 来自脑干或背根。多巴胺纤维的命运也将 进行评估。其他研究将评估功能能力 动物使用复杂的随机阶段模型来评估生理 回应。该技术由 PI 开发，提供了 定量衡量各部门之间的协调程度。在 同样的动物，再生纤维将被解剖学追踪并使用 GABA 和甘氨酸的特异性标记。