ENCODING TIME--DYNAMIC ANALYSIS OF BEHAVIOR

编码时间--行为动态分析

基本信息

批准号：
6186575
负责人：
AVIS H COHEN
金额：
$ 14.81万
依托单位：
UNIV OF MARYLAND, COLLEGE PARK
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-03-01 至 2002-04-30
项目状态：
已结题

项目摘要

Our research focuses on the production and control of locomotor behavior in the lamprey, with the goal of understanding behavior at the systems level. We propose three areas of investigation, two are continuations of previously begun projects, and one is new: I. Investigation of the structure and function of the spinal central pattern generator (CPG for locomotion, especially the intersegmental coordinating system. We will estimate functional coupling strengths in the CPG by fitting stochastic models to experimental data. Inhibitory baths of varying lengths will be used to study the relative strength of long versus short connections. Perturbations produced by bending the spinal cord with be used to help distinguish between ascending and descending coupling. Besides entraining the CPG, bending (forcing) elicts a slowly-decaying decrease in cycle period; we will search for a similar slow process in unforced data. We will extend the theoretical framework for entrainment by including long distance and non- relative-phase coupling; experimentally, we will determine how entrainment ranges vary with the number of active segments. Connectionist models of the CPG will be used to explore the dependence of intersegmental phase lags on the lengths and strengths of connections as well as the spatial variation in oscillator frequencies. We will analyze models of coupled heterogeneous populations of bursting neurons and explore the relevance of heterogeneity to intersegmental coordination. On a new experimental front, we will explore the use of optical dyes to record from groups of neurons in the CPG circuit, as well as to identify new classes of coordinating neurons. II. Normal locomotor behavior: integration of the components of the body to produce whole animal swimming. This is largely a continuation of work begun the last grant cycle. We will explore the role of the water in producing the traveling wave, the role of sensory inputs on the pattern, and the interaction between the brainstem and spinal cord. By combining all of these aspects of locomotor control, we are working toward a complete understanding of the production of an entire behavior in a vertebrate, and how all the parts of the organism and its environment combine and interact to produce that behavior. III. Longitudinal studies of the development and adaptability of the lamprey locomotor CPG. This is a new direction for the lab. The fictive swimming all sized of larval lampreys obtainable will be recorded, and the coupling strength among the segments estimated. If the motor pattern of the spinal cords of any size behaves very differently from the adults, new stochastic models will be used for the estimations. Also, we will raise animals under a variety of test to test the plasticity and adaptability of the CPG.

我们的研究重点是运动的产生和控制七鳃鳗的行为，目的是了解七鳃鳗的行为系统级别。我们提出三个调查领域，其中两个是先前开始的项目的延续，其中一个是新的：一、脊髓中枢结构与功能的考察模式生成器（用于运动的 CPG，尤其是节段间协调系统。我们将估计功能耦合强度通过将随机模型与实验数据拟合来计算 CPG。抑制性不同长度的浴槽将用于研究相对强度长连接与短连接。弯曲产生的扰动脊髓可用于帮助区分上行和上行下降耦合。除了夹带 CPG 之外，还可以弯曲（强制）导致周期周期缓慢衰减；我们将寻找一个非受迫数据中的类似缓慢过程。我们将扩展理论夹带框架，包括长途和非相对相位耦合；通过实验，我们将确定如何夹带范围随活性段的数量而变化。 CPG 的联结主义模型将用于探索段间相位滞后对长度和强度的依赖性连接以及振荡器频率的空间变化。我们将分析耦合异质群体的模型爆发神经元并探索异质性与部门间协调。在新的实验战线上，我们将探索使用光学染料来记录神经元组 CPG 电路，以及识别新的协调类别神经元。二.正常运动行为：各组件的整合身体产生整个动物的游泳。这很大程度上是一个继续工作从上一个拨款周期开始。我们将探索水在产生行波中的作用，感官的作用模式的输入以及脑干和脑干之间的相互作用脊髓。通过结合运动控制的所有这些方面，我们正在努力全面了解脊椎动物的整个行为，以及脊椎动物的所有部分如何有机体及其环境结合并相互作用以产生行为。三．发展和适应性的纵向研究七鳃鳗运动 CPG。这是实验室的一个新方向。这虚构的各种大小的七鳃鳗幼虫将在水中游泳记录，并估计各段之间的耦合强度。如果任何大小的脊髓的运动模式都表现得非常与成人不同，新的随机模型将用于估计。此外，我们还会饲养动物进行各种测试来测试 CPG的可塑性和适应性。