CORE--SERUM, LYMPHOCYTE AND TISSUE COLLECTION

核心——血清、淋巴细胞和组织采集

• 批准号: 6581152
• 负责人: Rishab K. Gupta
• 金额: $ 5.34万
• 依托单位: SAINT JOHN'S CANCER INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-15 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6581152
• 关键词: biomedical facility; cancer registry /resource; human tissue; lymphocyte; neoplastic cell; serum; tissue /cell preparation; tissue resource /registry; tumor antigens

DESCRIPTION (provided by applicant): This Core will serve as a central facility to collect and process blood for serum and lymphocytes, and tumor tissues for initiation of tissue culture cell lines and cryopreservation of tissue minces. This will be done according to the standard operating procedures (SOPs) to promote uniformity in handling and record-keeping of the samples received from the various centers participating in the trial as well as from the JWCI clinics. The centralized collection and processing of specimens will allow efficient use of materials, reduce the frequency of venipuncture, and allow maintenance of process uniformity, thus preserving the highest possible quality of the specimens to be used by leaders in various projects. The procedures will include the following: (1) Centralized processing of blood specimens received from JWCI and participating trial centers for serum and lymphocytes; (2) Cryopreservation and inventorying of the separated serum and lymphocytes into freezers at pre-assigned locations, and maintenance of appropriate records in computerized database; (3) Retrieval and distribution of cryopreserved specimens to leaders of various projects as needed; (4) Processing of tumor tissues for cryopreservation as tissue minces and initiation of primary cell lines; (5) Maintenance of tumor cell lines, and retrieval and distribution of cells to leaders of various projects; and (6) Target antigen purification from spent-culture medium, tumor cell extracts and urine of cancer patients according to already established procedures. The specimens upon receipt will be examined for physical appearance and checked for accuracy of the information and appropriateness of the accompanying forms. Information that will accompany the specimen will be verified with pathology reports. To maintain confidentiality, the specimens will be coded and appropriate records maintained at a central location.

描述（由申请人提供）：该核心将作为一个中心 收集和处理血清、淋巴细胞和肿瘤血液的设施 用于启动组织培养细胞系和冷冻保存的组织 组织切碎。这将根据标准操作来完成 程序（SOP），以促进处理和记录保存的统一 还从参与试验的各个中心收到了样本 来自 JWCI 诊所。集中收集、处理 标本将允许有效利用材料，减少频率 静脉穿刺，并允许维持过程的一致性，从而保留 各个领域的领导者使用的最高质量的样本 项目。程序将包括以下内容： (1) 集中 处理从 JWCI 和参与试验收到的血液样本 血清和淋巴细胞中心； (2) 冷冻保存和库存 将分离的血清和淋巴细胞放入预先指定位置的冰箱中， 并在计算机数据库中维护适当的记录； (3) 检索冷冻保存的标本并将其分发给各个领导人 根据需要进行项目； (4) 肿瘤组织的冷冻保存处理如下 组织切碎和原代细胞系的起始； (5)肿瘤的维持 细胞系，以及细胞的检索和分配给各种领导者 项目； (6) 从废培养基、肿瘤中纯化靶抗原 根据已经建立的癌症患者的细胞提取物和尿液 程序。收到样本后将进行物理检查 外观并检查信息的准确性和适当性 随附的表格。样本随附的信息将是 经病理报告证实。为了保密，样本 将进行编码并在中央位置维护适当的记录。