Genetic Analysis Facility

遗传分析设备

基本信息

批准号：
6440972
负责人：
SHAILENDRA BHANUBHAI PATEL
金额：
$ 13.75万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-05-01 至 2003-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6440972
关键词：
biomedical equipment purchase genetic techniques in situ hybridization molecular genetics nucleic acid sequence

项目摘要

DESCRIPTION (provided by applicant): The purpose of this proposal is to establish a Genetic Analysis Facility for the Medical University of South Carolina (MU SC). The key components of this are two ABI 3100 Genetic Analyzers, a Transgenomic WAVE Nucleic Acid Fragment Analysis System, one InTavis In Situ Pro in situ robot plus digital analysis system, and one high-speed bench top centrifuge. This Core will provide: 1. Rapid and high volume throughput of genotype analyses 2. Rapid and high volume screening for polymorphisms and mutations 3. Rapid and high-volume in situ analyses for gene expression patterns 4. Rapid and high volume DNA sequence analyses. The primary user group consists of 15 NIH-funded principal investigators with documented need. Another 49 investigators with NIH funding and 42 with other federal or state funding will also use the Genetic Analysis Facility. This facility is needed to bring state-of-the art technologies to the campus and allow investigators, whose research aims are heavily dependent upon the use of genetic analyses, to allow them to rapidly and efficiently execute these tasks. The requested instruments will enable NIH-funded investigators to apply state of the art technology to their analysis of large volumes of genetic data and to begin to translate this information into functional models at the cellular and molecular level.

描述（由申请人提供）：该提案的目的是为南方医科大学建立遗传分析设施卡罗莱纳州（MU SC）。其关键组件是两个 ABI 3100 Genetic 分析仪，一种转基因 WAVE 核酸片段分析系统，一种 InTavis In Situ Pro 原位机器人加数字分析系统，以及一台高速台式离心机。该核心将提供： 1. 快速、高通量的基因型分析 2. 快速、大量筛选多态性和突变 3. 基因表达模式的快速、大容量原位分析 4. 快速、大容量 DNA 序列分析。主要用户组由 15 名 NIH 资助的主要研究人员组成记录的需要。另外 49 名研究人员获得 NIH 资助，42 名研究人员获得其他资助联邦或州的资金也将使用遗传分析设施。这需要设施将最先进的技术引入校园，并且允许研究人员，其研究目标在很大程度上取决于使用遗传分析，使他们能够快速有效地执行这些任务。所请求的文书将使 NIH 资助的研究人员能够申请国家将先进技术应用于分析大量遗传数据并开始将这些信息转化为细胞和功能模型分子水平。