MOLECULAR MECHANISMS OF GROWTH CONE GUIDANCE

生长锥引导的分子机制

基本信息

批准号：
6637672
负责人：
AKIRA CHIBA
金额：
$ 28.98万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-05-01 至 2004-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6637672
关键词：
Drosophilidae axon cell adhesion molecules chemical binding dendrites developmental neurobiology growth cones invertebrate embryology molecular shape motor neurons muscle cells neuronal guidance single cell analysis synapses synaptogenesis tissue /cell culture

项目摘要

DESCRIPTION (Adapted from the Applicant's Abstract): The goal of this project is to test the validity of two general hypotheses: (1) the growth cone reprogramming hypothesis, and (2) the unique dendritic guidance hypothesis. According to the first hypothesis, axon guidance occurs through an interactive reprogramming model, in which a growth cone must encounter specific molecular cues early during its navigation so that it can undergo a reprogramming process that enables it to properly respond to cues presented subsequently. The project adopts in vivo single cell analysis, while taking the CNS midline as one example of early guidance cues that may be responsible for growth cone reprogramming. The second hypothesis is a model to explain why dendrites and axons grow differently. According to this hypothesis, axons and dendrites grow differently because they use a different set of growth cone receptor/signaling molecules. A neuron is capable of targeting molecules differentially to its axon or dendrites, thereby allowing the differential expression of receptors at each site. Additional possibilities are that dendritic growth may depend on axonal guidance, or that dendritic growth cones may be more susceptible to neuronal activity. This project will begin to tease apart the unique dendritic guidance hypothesis by using in vivo single cell analysis of dendritic development as compared to axonal development. It is anticipated that the present study will provide a better understanding of operational principles of growth cone guidance.

描述（改编自申请人的摘要）：该项目的目标是测试两个一般假设的有效性：（1）生长锥重编程假设，以及（2）独特的树突状引导假设。根据第一个假设，轴突引导是通过交互式进行的重编程模型，其中生长锥必须遇到特定的分子导航期间的早期提示，以便可以进行重新编程的过程这使其能够正确响应随后提出的提示。项目采用体内单细胞分析，同时将CNS中线作为一个可能负责增长锥的早期指导提示的示例重新编程。第二个假设是解释为什么树突和为什么轴突的生长不同。根据这一假设，轴突和树突生长以不同的方式使用，因为它们使用了不同的生长锥受体/信号传导分子。神经元能够将分子靶向其靶向轴突或树突，从而允许受体的差异表达每个站点。其他可能性是树突状增长可能取决于轴突引导或树突生长锥可能更容易受到神经元活性。这个项目将开始逗弄独特的树突状通过使用体内单细胞分析树突状的指导假设与轴突发育相比。预计目前的研究将更好地了解生长锥指导。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A single growth cone is capable of integrating simultaneously presented and functionally distinct molecular cues during target recognition.

单个生长锥能够在目标识别过程中整合同时呈现且功能不同的分子线索。

DOI：
10.1523/jneurosci.19-12-04899.1999
发表时间：
1999
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
0
作者：
Rose,D;Chiba,A
通讯作者：
Chiba,A

Toll, a muscle cell surface molecule, locally inhibits synaptic initiation of the RP3 motoneuron growth cone in Drosophila.

Toll 是一种肌肉细胞表面分子，可局部抑制果蝇 RP3 运动神经元生长锥的突触起始。